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Vasoactive intestinal polypeptide immunoreactive neurons in the primary visual cortex of the cat

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Journal of Neurocytology

Summary

When cat visual cortex (area 17) is reacted with an antibody to vasoactive intestinal polypeptide (VIP) a variety of neuronal types is labelled. Many of the labelled neurons are bipolar in form and are most common in layers II and III, although significant numbers of bipolar neurons are also encountered in layer V. Multipolar cells are also labelled. These are most frequent in layer IV and have a variety of shapes. In layer I, the labelled cells are of three varieties, i.e. horizontal bipolar cells, horizontal bitufted cells and multipolar neurons, while in layer VI the few VIP-positive neurons are horizontal bipolar cells. This suggests that all of the VIP-labelled neurons in cat area 17 are non-pyramidal in form, and this has been confirmed by electron microscopy.

In these preparations, axon terminals are also labelled and under the light microscope it can be seen that these terminals occur both within the neuropil and around the cell bodies of some neurons, particularly neurons in layers II and III. Electron microscopy has shown that all of the labelled axon terminals form symmetric synapses and that those in the neuropil synapse with the shafts of smooth dendrites. These axodendritic synapses account for about 90% of the synapses formed by the labelled axon terminals. The remainder of the labelled axon terminals synapse with the cell bodies of pyramidal neurons.

Parallels are drawn between these results and those previously obtained by examining those neuronal elements labelled with VIP antibodies in rat visual cortex.

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Author notes

  1. Azarias N. Karamanlidis

    Present address: Department of Anatomy, Veterinary School, University of Thessalonika, Greece

Authors and Affiliations

  1. Department of Anatomy, Boston University School of Medicine, 80 East Concord Street, 02118, Boston, MA, USA

    Alan Peters, Douglas L. Meinecke & Azarias N. Karamanlidis

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  1. Alan Peters
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  2. Douglas L. Meinecke
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  3. Azarias N. Karamanlidis
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Peters, A., Meinecke, D.L. & Karamanlidis, A.N. Vasoactive intestinal polypeptide immunoreactive neurons in the primary visual cortex of the cat. J Neurocytol 16, 23–38 (1987). https://doi.org/10.1007/BF02456695

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  • DOI: https://doi.org/10.1007/BF02456695

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