Molecular and General Genetics MGG

, Volume 248, Issue 1, pp 52–58 | Cite as

Promoters of thephr gene inEscherichia coli K-12

  • Chuping Ma
  • Claud S. Rupert
Original Paper


We have identified two promoters of theEscherichia coli phr gene by DNA deletion mapping, S1 mapping of transcripts and sequence homology. The weaker promoter, P2, located approximately 530 bp upstream from the start codon, extends beyond the previously known nucleotide sequence. The stronger, P1, lies 90 bp from the gene and is distinct from three previously described promoter-like sequences nearby. β-Galactosidase production from a plasmid-borne gene, promoted by a synthetic copy of P1, increases after DNA damage, but the increase does not depend on the SOS-box-like sequences normally present in the vicinity of P1. Induction still requires intactrecA andlexA genes, and also intactsulA

Key words

phr Photolyase DNA repair sulA 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Chuping Ma
    • 1
  • Claud S. Rupert
    • 1
  1. 1.Program in Molecular and Cell BiologyThe University of Texas at Dallas, Box 830688RichardsonUSA

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