Advertisement

Molecular and General Genetics MGG

, Volume 248, Issue 1, pp 52–58 | Cite as

Promoters of thephr gene inEscherichia coli K-12

  • Chuping Ma
  • Claud S. Rupert
Original Paper

Abstract

We have identified two promoters of theEscherichia coli phr gene by DNA deletion mapping, S1 mapping of transcripts and sequence homology. The weaker promoter, P2, located approximately 530 bp upstream from the start codon, extends beyond the previously known nucleotide sequence. The stronger, P1, lies 90 bp from the gene and is distinct from three previously described promoter-like sequences nearby. β-Galactosidase production from a plasmid-borne gene, promoted by a synthetic copy of P1, increases after DNA damage, but the increase does not depend on the SOS-box-like sequences normally present in the vicinity of P1. Induction still requires intactrecA andlexA genes, and also intactsulA

Key words

phr Photolyase DNA repair sulA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aiba H, Adhya S, de Crombrugghe B (1981) Evidence for two functionalgal promoters in intactEscherichia coli cells. J Biol Chem 256:11905–11910PubMedGoogle Scholar
  2. Alcorn JL, Rupert CS (1990) Regulation of photolyase inEscherichia coli K-12 during adenine deprivation. J Bacteriol 172:6885–6891PubMedGoogle Scholar
  3. Band L, Yansura DG, Henner DJ (1983) Construction of a vector for cloning promoters inBacillus substilis. Gene 26: 313–315PubMedCrossRefGoogle Scholar
  4. Bertrand-Berggraf E, Oertel P, Schnarr M, Daune M, Grainger-Schnarr M (1989) Effect of induction of SOS response on expression of pBR322 genes and on plasmid copy number. Plasmid 22: 163–168CrossRefGoogle Scholar
  5. Brosius J (1984) Plasmid vectors for the selection of promoters. Gene 27: 151–160PubMedCrossRefGoogle Scholar
  6. Casadaban MJ, Chou J (1984) In vivo formation of gene fusions encoding hybrid β-galactosidase proteins in one step with a transposable Mulac transducing phage. Proc Natl Acad Sci USA 81: 535–539PubMedCrossRefGoogle Scholar
  7. Castellazzi M, George J, Buttin G (1972) Prophage induction and cell division inE. coli: I. Further characterization of the thermo-sensitive mutationtif-1 whose expression mimics the effect of UV irradiation. Mol Gen Genet 119: 139–152PubMedCrossRefGoogle Scholar
  8. Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic mini plasmid. J Bacteriol 134: 1141–1156PubMedGoogle Scholar
  9. DeWitt SK, Adelberg EA (1962) The occurrence of a genetic transposition in a strain ofEscherichia coli. Genetics 47: 577–585Google Scholar
  10. Ebina Y, Kishi F, Miki T, Kagamiyama H, Nakazawa T, Nakazawa A (1981) The nucleotide sequence surrounding the promoter region of colicin E1 gene. Gene 15: 119–126PubMedCrossRefGoogle Scholar
  11. George J, Castellazzi M, Buttin G (1975). Prophage induction and cell division inE. coli. III. MutationssfiA andsfiB restore division intif andlon strains and permit the expression of mutator properties oftif. Mol Gen Genet 140: 309–332PubMedGoogle Scholar
  12. Gottesman S, Halpern E, Trisler P (1981) Kole ofsulA andsulB in filamentation bylon mutants ofEscherichia coli K-12. J Bacteriol 148: 265–273PubMedGoogle Scholar
  13. Harm W (1969) Biological determination of the germicidal activity of sunlight. Radiat Res 40: 63–69PubMedGoogle Scholar
  14. Horii T, Ogawa T, Ogawa H (1980) Organization of therecA gene ofEscherichia coli. Proc Natl Acad Sci USA 77: 313–317PubMedCrossRefGoogle Scholar
  15. Ihara M, Yamamoto K, Ohnishi T (1987) Induction ofphr gene expression by irradiation of ultraviolet light inEscherichia coli. Mol Gen Genet 209: 200–202PubMedCrossRefGoogle Scholar
  16. Lederberg J (1951) Genetic studies with bacteria. In: Dunn LC (ed.) Genetics in the 20th century. Essays on the progress of genetics during its first 50 years. Macmillan, New York, pp 263–289Google Scholar
  17. Little JW, Mount DW (1982) The SOS regulatory system ofE. coli. Cell 29: 11–22PubMedCrossRefGoogle Scholar
  18. Lorence MC, Rupert CS (1983) Convenient construction ofrecA deletion derivatives ofEscherichia coli. J Bacteriol 156: 458–459PubMedGoogle Scholar
  19. Lorence MC, Alcorn JL, Rupert CS (1984) Construction of an improved maxicell strain for the identification of recombinant plasmid encoded proteins. Basic Life Sci 30: 955Google Scholar
  20. Lorence MC, Maika SD, Rupert CS (1990) Physical analysis ofphr gene transcription inEscherichia coli K-12. J Bacteriol 172: 6551–6556PubMedGoogle Scholar
  21. Maguin E, Lutkenhaus J, D'Ari R (1986) Reversibility of SOS-associated division inhibition inEscherichia coli. J Bacteriol 166: 733–738PubMedGoogle Scholar
  22. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  23. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  24. Misusawa S, Gottesman S (1983) Protein degradation inEscherichia coli: thelon gene controls the stability ofsulA protein. Proc Nat Acad Sci USA 80: 358–362CrossRefGoogle Scholar
  25. Mount DW, Low KB, Edmiston SJ (1972) Dominant mutations (lex) inEscherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J Bacteriol 112: 886–893PubMedGoogle Scholar
  26. Nishioka N, Harm W (1972) Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. IX. Excess production of photoreactivating enzyme inE. coli Bs−1-160 under different growth conditions and its suppression by adenine. Mutat Res 16: 121–131PubMedGoogle Scholar
  27. Pahel G, Rothestein D, Magasanik B (1982) ComplexglnA-glnC-glnG operon ofEscherichia coli. J Bacteriol 150: 202–213PubMedGoogle Scholar
  28. Payne N, Sancar A (1989) ThelexA protein does not bind specifically to the SOS-box-like sequences immediately 5′ to thephr gene. Mutat Res 218: 207–210PubMedGoogle Scholar
  29. Rupert CS (1975) Enzymatic photoreactivation: Overview. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA. Part A. Plenum, New York pp 73–87Google Scholar
  30. Sancar A, Rupert CS (1978) Correction of the map location for thephr gene inEscherichia coli K-12. Mutat Res 51: 139–143Google Scholar
  31. Sancar A, Sancar GB (1988) DNA repair enzymes. Annu Rev Biochem 57: 29–67PubMedCrossRefGoogle Scholar
  32. Sancar GB, Smith FW, Sancar A (1983) Identification and amplification of theE. coli phr gene product. Nucleic Acid Res 11: 6667–6678PubMedGoogle Scholar
  33. Sancar A, Franklin KA, Sancar GB (1984a)Escherichia coli DNA photolyase stimulates UvrABC exision nucleasein vitro. Proc Natl Acad Sci USA 81: 7397–7401PubMedCrossRefGoogle Scholar
  34. Sancar GB, Smith FW, Lorence MC, Rupert CS, Sancar A (1984b) Sequences of theEscherichia coli photolyase gene and protein. J Biol Chem 259: 6033–6038PubMedGoogle Scholar
  35. Sanger F, Ruklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Nat Acad Sci USA 74: 5463–5467PubMedCrossRefGoogle Scholar
  36. Simons RW, Houman F, Kleckner N (1987) Improved single and multicopylac-based cloning vectors for protein and operon fusions. Gene 53: 85–96PubMedCrossRefGoogle Scholar
  37. Tyrrell RM (1973) Suppression of photoreactivating enzyme production inEscherichia coli growth under anaerobic conditions. J Bacteriol 115: 450–452PubMedGoogle Scholar
  38. Tyrrell RM, Moss SH, Davies DTG (1972) The variation in photoreactivating enzyme activity as a function of the stage of growth of three K-12 strains ofEscherichia coli. Mutat Res 16: 345–352PubMedGoogle Scholar
  39. Walker G (1984) Mutagenesis and inducible repairs to deoxyribonucleic acid damage inEscherichia coli. Microbiol Rev 48: 60–93PubMedGoogle Scholar
  40. Yamamoto K, Fujinara Y, Shiagawa H (1983) Evidence that theph + gene enhances the ultraviolet resistance ofEscherichia coli recA strains in the dark. Mol Gen Genet 192: 282–284PubMedCrossRefGoogle Scholar
  41. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Chuping Ma
    • 1
  • Claud S. Rupert
    • 1
  1. 1.Program in Molecular and Cell BiologyThe University of Texas at Dallas, Box 830688RichardsonUSA

Personalised recommendations