Skip to main content

Advertisement

Log in

Characterization ofBorrelia burgdorferi sensu lato strains isolated fromIxodes ricinus in Mecklenburg-Vorpommern, Germany

  • Original Investigation
  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence ofBorrelia burgdorferi-infected ticks. A total of 17B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal antibodies against different epitopes of the outer surface protein A (OspA). Except for one, all strains could be classified using this system. The majority of strains belonged to theB. garinii-associated OspA serotypes 3, 5 and 6. Three isolates were classified as OspA serotype 2 (B. afzelii).B. burgdorferi sensu stricto strains (Ospa serotype 1) as well asB. garinii-associated OspA serotype 4 were not present.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Adam T, Gassmann GS, Rasiah C, Göbel UB (1991) Phenotypic and genotypic analysis ofBorrelia burgdorferi isolates from various sources. Infect Immun 59:2579–2585

    PubMed  CAS  Google Scholar 

  2. Anderson JF (1991) Epizootiology of Lyme borreliosis. Scand J Infect Dis 77:23–24

    CAS  Google Scholar 

  3. Baranton G, Postic D, Saint Girons I, Boerlin P, Piffaretti JC, Assous M, Grimont PAD (1992) Delineation ofBorrelia burgdorferi sensu stricto,Borrelia garinii sp. nov., and group VS 461 associated with Lyme borreliosis. Int J Syst Bacteriol 42:378–383

    Article  PubMed  CAS  Google Scholar 

  4. Bunikis J, Olsén B, Westman G, Bergström S (1995) Variable serum immunoglobulin response against differentBorrelia burgdorferi sensu lato species in a population at risk for and patients with Lyme disease. J Clin Microbiol 33:1473–1478

    PubMed  CAS  Google Scholar 

  5. Comstock LE, Fikrig E, Shoberg RJ, Flavell RA, Thomas DD (1993) A monoclonal antibody to OspA inhibits association ofBorrelia burgdorferi with human endothelial cells. Infect Immun 61:423–431

    PubMed  CAS  Google Scholar 

  6. Eiffert H, Ohlenbusch A, Fehling W, Lotter H, Thomssen R (1992) Nucleotide sequence of theospAB operon of aBorrelia burgdorferi strain expressing OspA but not OspB. Infect Immun 60:1864–1868

    PubMed  CAS  Google Scholar 

  7. Fikrig E, Barthold SW, Kantor FS, Flavell RA (1990) Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science 250:553–556

    PubMed  CAS  Google Scholar 

  8. Kawabata H, Masuzawa T, Yanagihara Y (1993) Genomic analysis ofBorrelia japonica sp. nov. isolated fromIxodes ovatus. Microbiol Immunol 37:843–848

    PubMed  CAS  Google Scholar 

  9. Lebech AM, Hansen K, Wilske B, Theisen M (1994) Taxonomic classification of 29Borrelia burgdorferi strains isolated from patients with Lyme borreliosis: a comparison of five different phenotypic and genotypic typing schemes. Med Microbiol Immunol 183:325–341

    Article  PubMed  CAS  Google Scholar 

  10. Preac-Mursic V, Wilske B, Schierz G, Holmburger M, Süss E (1987) In vitro and in vivo susceptibility ofBorrelia burgdorferi. Eur J Clin Microbiol 6:424–426

    Article  Google Scholar 

  11. Preac-Mursic V, Wilske B, Patsouris E, Jauris S, Will G, Soutschek E, Reinhardt S, Lehnert G, Klockmann U, Mehraein P (1992) Active immunization with pC protein ofBorrelia burgdorferi protects gerbils againstBorrelia burgdorferi infection. Infection 20:342–349

    Article  PubMed  CAS  Google Scholar 

  12. Sadziene A, Thomas DD, Barbour AG (1995)Borrelia burgdorferi mutant lacking Osp: biological and immunological characterization. Infect Immun 63:1573–1580

    PubMed  CAS  Google Scholar 

  13. Schaible UE, Kramer MD, Eichmann K, Modolell M, Museteanu C, Simon MM (1990) Monoclonal antibodies specific for the outer surface protein A (OspA) prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proc Natl Acad Sci USA 87:3768–3772

    Article  PubMed  CAS  Google Scholar 

  14. Steere AC (1989) Medical progress—Lyme disease. N Engl J Med 321:586–596

    Article  PubMed  CAS  Google Scholar 

  15. Thomas DD, Comstock LE (1989) Interaction of Lyme disease spirochetes with cultured eucaryotic cells. Infect Immun 57: 1324–1326

    PubMed  CAS  Google Scholar 

  16. Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour AG, Kramer M (1988) Antigenetic variability ofBorrelia burgdorferi. Ann NY Acad Sci 539:126–143

    PubMed  CAS  Google Scholar 

  17. Wilske B, Preac-Mursic V, Göbel UB, Graf B, Jauris S, Soutschek E, Schwab E, Zumstein G (1993) An OspA serotyping system forBorrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J Clin Microbiol 31:340–350

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lottmann, H., Wilske, B. & Herrmann, H. Characterization ofBorrelia burgdorferi sensu lato strains isolated fromIxodes ricinus in Mecklenburg-Vorpommern, Germany. Med Microbiol Immunol 184, 181–184 (1996). https://doi.org/10.1007/BF02456133

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02456133

Key words

Navigation