Molecular and Cellular Biochemistry

, Volume 110, Issue 2, pp 155–159 | Cite as

Amino acid metabolism in several tissues of the obese Zucker rat as indicated by the tissue accumulation of α-amino[1-14C]isobutyrate

  • Margarida Domènech
  • Francisco J. López-Soriano
  • Neus Carbó
  • Josep M. Argilés


Measurements of the tissue accumulation of α-amino[1-14C]isobutyrate [1-14C]AIB) in lean (+/?) and obese (fa/fa) Zucker rats showed an augmented tissue/plasma ratio in the liver of the obese animals. In contrast, brown adipose tissue AIB accumulation was lower in the fa/fa animals. In response to a 24h starvation period AIB accumulation was significantly elevated in the liver and plasma of the lean animals and was unchanged in the liver of the fa/fa animals. The circulating concentration of alanine and branched-chain amino acids was elevated in the fa/fa animals as compared to their lean counterparts. These observations suggest that amino acid uptake is not involved in the impaired muscle development observed in the obese Zucker rat and that the ability of brown adipose tissue for amino acid utilization is decreased in the obese animals suggesting that this may partially explain the impaired thermoregulatory capacity observed in brown adipose tissue of obese Zucker rats.

Key words

Zucker rat amino acid metabolism skeletal muscle obesity 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Margarida Domènech
    • 1
  • Francisco J. López-Soriano
    • 1
  • Neus Carbó
    • 1
  • Josep M. Argilés
    • 1
  1. 1.Unitat de Bioquímica i Biologia Molecular B, Department de Bioquímica i Fisiologia, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

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