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Detection of a CpA methylase in an insect system: characterization and substrate specificity

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Abstract

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependance similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

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Author notes

  1. Chalasani Devajyothi

    Present address: Department of Molecular Biology, NN1-26, The Cleveland Clinic, Foundation, 9500, Euclid Avenue, OH-44195, USA

Authors and Affiliations

  1. Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012, Bangalore, India

    Chalasani Devajyothi & Vani Brahmachari

  2. ICMR Centre for Genetics and Cell Biology, Indian Institute of Science, 560 012, Bangalore, India

    Chalasani Devajyothi & Vani Brahmachari

Authors
  1. Chalasani Devajyothi
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  2. Vani Brahmachari
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Devajyothi, C., Brahmachari, V. Detection of a CpA methylase in an insect system: characterization and substrate specificity. Mol Cell Biochem 110, 103–111 (1992). https://doi.org/10.1007/BF02454187

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  • DOI: https://doi.org/10.1007/BF02454187

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