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Chromosoma

, Volume 102, Supplement 1, pp S107–S113 | Cite as

Control of DNA synthesis genes in budding yeast: involvement of the transcriptional modulatorMOT1 in the expression of the DNA polymerase α gene

  • Simonetta Piatti
  • Remo Tazzi
  • Antonella Pizzagalli
  • Paolo Plevani
  • Giovanna Lucchini
Article

Abstract

Periodic transcription during the cell cycle of the budding yeast DNA polymerase α gene (POLI) requires the cis-acting element 5′ ACGCGT 3′, which has been found in the 5′ non-coding region of all the DNA synthesis genes analyzed so far. Search for trans-acting mutations affectingPOL1 expression led to the isolation of the temperature-sensitivereg1033 mutant, that showed increased levels of both DNA polymerase α and δ gene transcripts. Cloning of theREG1033 gene demonstrated that it is essential for cell viability and required for proper expression of thePOL1 gene. DNA sequence comparison established that theREG1033 gene is identical toMOT1, a gene encoding a presumptive DNA helicase which modulates transcription of several yeast genes.

Keywords

Upstream Activate Sequence lacZ Fusion Strain CG378 POLl mRNA POLl Transcription 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Simonetta Piatti
    • 1
  • Remo Tazzi
    • 1
  • Antonella Pizzagalli
    • 1
  • Paolo Plevani
    • 1
  • Giovanna Lucchini
    • 1
    • 2
  1. 1.Dipartimento di Genetica e di Biologia dei MicrorganismiUniversità degli Studi di MilanoMilanoItaly
  2. 2.Istituto di GeneticaUniversità di SassariSassariItaly

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