Separation of phosphoribulokinase from enzymes of the calvin cycle inHydrogenomonas eutropha H 16
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Phosphoribulokinase ofHydrogenomonas eutropha, H 16 was maximally induced during autotrophic growth. The enzyme was not associated with fructose biphosphatase or ribulose diphosphate carboxylase in contrast to reports in other bacteria. The three activities were separated during sucrose density gradient centrifugation, gel filtration and DEAE-cellulose chromatography. The molecular weights of phosphoribulokinase, fructose biphosphatase, ribulose diphosphate carboxylase and ribose 5-phosphate isomerase were estimated to be 237000, 187000, 505000 and 50000, respectiveley.
KeywordsFructose Isomerase Calvin Cycle Ribulose Thiobacillus
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