Summary
Phosphoribulokinase ofHydrogenomonas eutropha, H 16 was maximally induced during autotrophic growth. The enzyme was not associated with fructose biphosphatase or ribulose diphosphate carboxylase in contrast to reports in other bacteria. The three activities were separated during sucrose density gradient centrifugation, gel filtration and DEAE-cellulose chromatography. The molecular weights of phosphoribulokinase, fructose biphosphatase, ribulose diphosphate carboxylase and ribose 5-phosphate isomerase were estimated to be 237000, 187000, 505000 and 50000, respectiveley.
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References
Bergmeyer, H. U.: Methoden der enzymatischen Analyse, 2. Aufl., Bd. 1, S. 439–440. Weinheim/Bergstr.: Verlag Chemie 1970
Gibson, J., Hart, B. A.: Localization and characterization of Calvin cycle enzymes inChromatium strain D. Biochemistry8, 2737–2741 (1969)
Gottschalk, G., Eberhardt, U., Schlegel, H. G.: Verwertung von Fructose durchHydrogenomonas H 16 (L). Arch. Mikrobiol.48, 95–108 (1964)
Hart, B. A., Gibson, J.: Ribulose 5-phosphate kinase fromChromatium sp. strain D. Arch. Biochem. Biophys.144, 308–321 (1971)
Joint, I. R., Morris, I., Fuller, R. C.: Purification of a complex of alkaline fructose 1,6-diphosphatase and phosphoribulokinase fromRhodospirillum rubrum. J. biol. Chem.247, 4833–4838 (1972)
Kuehn, G. D., McFadden, B. A.: Ribulose 1,5-diphosphate carboxylase fromHydrogenomonas facilis. I. Purification, metallic ion requirements, inhibition and kinetic constants. Biochemistry8, 2394–2402 (1969)
Lowry, C. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
MacElroy, R. D., Johnson, E. J., Johnson, M. K.: Characterization of ribulose diphosphate carboxylase and phosphoribulokinase fromThiobacillus thioparus andThiobacillus neapolitanus. Arch. Biochem. Biophys.127, 310–316 (1968)
McElroy, R. D., Mack, H. M., Johnson, E. M.: Properties of phosphoribulokinase fromThiobacillus neapolitanus. J. Bact.112, 532–538 (1972)
Martin, R. G., Ames, B. N.: A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J. biol. Chem.235, 1372–1379 (1961)
McFadden, B., Tu, C. C. L.: Regulation of autotrophic and heterotrophic carbon dioxide fixation inHydrogenomonas facilis. J. Bact.93, 886–893 (1967)
Pontremoli, S., Mangiarotti, G.: A simple method for the preparation ofd-ribulose 5-phosphate. J. biol. Chem.237, 643–645 (1962)
Schlegel, H. G., Kaltwasser, H., Gottschalk, G.: Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: wachstumsphysiologische Untersuchungen. Arch. Mikrobiol.39, 209–222 (1961)
Springgate, C. F., Stachow, C. S.: Fructose 1,6-diphosphatase fromRhodopseudomonas palustris. I. Purification and properties. Arch. Biochem. Biophys.152, 1–12 (1972)
Wilde, E.: Untersuchungen über Wachstum und Speicherstoffsynthese vonHydrogenomonas. Arch. Mikrobiol.,43, 109–137 (1962)
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Abdelal, A.T.H., Schlegel, H.G. Separation of phosphoribulokinase from enzymes of the calvin cycle inHydrogenomonas eutropha H 16 . Arch. Microbiol. 95, 139–143 (1974). https://doi.org/10.1007/BF02451755
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DOI: https://doi.org/10.1007/BF02451755