Inhibition by nisin of K+ uptake in a sensitivePediococcus sp. strain
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A nisin-sensitive strain ofPediococcus sp possessed an uptake system for K+ which was apparently dependent on metabolic energy and ATPase activity. K+ uptake rate was dependent on the glucose and K+ concentrations and showed approximately Michaelis-Menten kinetics with respect to both of these variables with Kt values of 1.2 mM and 599 μM respectively.
The presence of nisin inhibited K+ uptake with the percentage inhibition proportional to the nisin activity,. Total inhibition occurred at between 4.5 and 5.0 IU ml−1 and the MIC was approximately 0.6 IU ml−1.
KeywordsFermentable Substrate Pipe Buffer 50mM Glucose Nisin Activity Half Maximal Stimulation
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