Biotechnology Techniques

, Volume 6, Issue 6, pp 561–564 | Cite as

Inhibition by nisin of K+ uptake in a sensitivePediococcus sp. strain

  • C. White
  • N. L. Morgan
  • J. S. Rockey
Article

Summary

A nisin-sensitive strain ofPediococcus sp possessed an uptake system for K+ which was apparently dependent on metabolic energy and ATPase activity. K+ uptake rate was dependent on the glucose and K+ concentrations and showed approximately Michaelis-Menten kinetics with respect to both of these variables with Kt values of 1.2 mM and 599 μM respectively.

The presence of nisin inhibited K+ uptake with the percentage inhibition proportional to the nisin activity,. Total inhibition occurred at between 4.5 and 5.0 IU ml−1 and the MIC was approximately 0.6 IU ml−1.

Keywords

Fermentable Substrate Pipe Buffer 50mM Glucose Nisin Activity Half Maximal Stimulation 

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References

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Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • C. White
    • 1
  • N. L. Morgan
    • 1
  • J. S. Rockey
    • 1
  1. 1.Department of BiotechnologySouth Bank PolytechnicLondon

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