Biotechnology Techniques

, Volume 6, Issue 6, pp 533–538 | Cite as

Purification of lipases fromChromobacterium viscosum: Is there a high molecular weight lipase?

  • M. A. Taipa
  • M. R. Aires-Barros
  • J. M. S. Cabral
Article

Abstract

A commercial lipolytic preparation ofChromobacterium viscosum has been separated by gel filtration on a sephacryl 200 HR column. Two lipolytic active fractions were obtained. PAGE, SDS-PAGE and IEF analysis were performed on both the mixture and the two lipolytic fractions. The results do not completely agree with those reported in the literature, suggesting that the high molecular weight lipase is probably an aggregate of lipases contained in the low molecular weight peak.

Keywords

Lipase Triolein Isobe Cabral Lipase Production 
PAGE

Polyacrylamide Gel Electrophoresis

SDS-PAGE

Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis

IEF

Isoelectric Focusing

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Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • M. A. Taipa
    • 1
  • M. R. Aires-Barros
    • 1
  • J. M. S. Cabral
    • 1
  1. 1.Laboratório de Engenharia BioquímicaInstituto Superior TécnicoLisboaPortugal

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