Biotechnology Techniques

, Volume 6, Issue 5, pp 473–476 | Cite as

Efficient protoplast regeneration ofBacillus thuringiensis andAgrobacterium tumefaciens

  • U. S. Puntambekar
  • D. V. Gokhale
  • P. K. Ranjekar
Article

Summary

Treatment ofBacillus thuringiensis andAgrobacterium tumefaciens taken from the early growth phase (8 h) with lysozyme at 1 mg/ml gave 90–99% protoplast formation and 10–12% protoplast regeneration on the minimal medium in absence of plasma expander (Bovine serum albumin). Enhanced fusion frequency was obtained when protoplasts from 8 h grown cells were used for fusion experiments.

Keywords

Lysozyme Protoplast Fusion Plasma Expander Grown Cell Intergeneric Hybrid 

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References

  1. Puntambekar, U.S. and Ranjekar, P.K. (1989). Biotechnol. Lett. 11, 717–722.CrossRefGoogle Scholar
  2. Tenmeyer, K.B. (1987). J. Gen. Microbiol. 133, 503–506.Google Scholar
  3. Weiss, R.L. (1976) J. Bacteriol. 128, 668–670.Google Scholar

Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • U. S. Puntambekar
    • 1
  • D. V. Gokhale
    • 1
  • P. K. Ranjekar
    • 1
  1. 1.Division of Biochemical SciencesNational Chemical LaboratoryPuneIndia

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