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Influence of formaldehyde in control of bacterial and fungal contaminants in plant cell cultures: Its effect on growth and secondary metabolite production

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Biotechnology Techniques

Summary

Influence of formaldehyde on growth and secondary metabolite production inin vitro grown tissues of pyrethrum, capsicum and carrot were studied. Formaldehyde concentration above 0.025% completely inhibited the growth of addedBacillus andAspergillus in the culture medium. Formaldehyde up to 0.025% level caused slight inhibition in the growth of pyrethrum callus. Callus subjected to 0.05, 0.1 and 0.2% formaldehyde enhanced the level of pyrethrin production in pyrethrum callus, whereas production of phenolics was lower in all the treatments. Growth of carrot callus and anthocyanin production was not inhibited up to a formaldehyde concentration of 0.04%. Similarly, the production of capsaicin in immobilised cell cultures ofCapsicum frutescens was not inhibited up to 0.04% level of formaldeyde. The results demonstrate the usefulness of formaldehyde to control contamination in plant tissue culture.

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Authors and Affiliations

  1. Autotrophic Cell Culture Discipline, Central Food Technological Research Institute, 570 013, Mysore, India

    C. Nirmala, G. Suvarnalatha, G. A. Ravishankar & L. V. Venkataraman

Authors
  1. C. Nirmala
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  2. G. Suvarnalatha
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  3. G. A. Ravishankar
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  4. L. V. Venkataraman
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Nirmala, C., Suvarnalatha, G., Ravishankar, G.A. et al. Influence of formaldehyde in control of bacterial and fungal contaminants in plant cell cultures: Its effect on growth and secondary metabolite production. Biotechnol Tech 6, 463–468 (1992). https://doi.org/10.1007/BF02447490

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  • DOI: https://doi.org/10.1007/BF02447490

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