Summary
A simple nucleotide sequence analysis method for the detection of frame-shift sequencing errors is presented. It is based on the fact that the base content of the three positions of the codons in protein-coding. DNA-sequences varies in a predictable manner correlated to the overall G+C content. A simple computer program is needed to perform this analysis.
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Abbreviations
- Bti :
-
Bacillus thuringiensis subsp.isrealensis
- kbp:
-
1,000 base pairs
- EMBL:
-
European Molecular Biology Laboratory
- ORF:
-
open reading frame
References
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Andrup, L., Damgaard, J., Laursen, B. et al. Using the relationship between nucleotide sequence and codon usage for the detection of frame-shift sequencing errors in bacterial genes. Biotechnol Tech 6, 461–462 (1992). https://doi.org/10.1007/BF02447489
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DOI: https://doi.org/10.1007/BF02447489