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A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor

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Biotechnology Techniques

Summary

A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view.

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Authors and Affiliations

  1. Unitat d'Enginyeria Química. Facultat de Ciències. Institut d'Enginyeria Bioquímica (UAB-CSIC), Universitat Autònoma de Barcelona, 08193, Bellaterra, (Spain)

    J. M. Serrat, G. Caminal, F. Gòdia, C. Solà & J. López-Santín

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  1. J. M. Serrat
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  2. G. Caminal
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  3. F. Gòdia
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  4. C. Solà
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  5. J. López-Santín
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Serrat, J.M., Caminal, G., Gòdia, F. et al. A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor. Biotechnol Tech 6, 451–454 (1992). https://doi.org/10.1007/BF02447487

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  • DOI: https://doi.org/10.1007/BF02447487

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