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Application of a novel PRODUCTIVTM CM column in egg-white protein separation: Effect of high flow-rate on resolution and productivity

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Biotechnology Techniques

Summary

A preparative PRODUCTIVTM CM, a new coherent ion-exchanger, column (250×10 mm ID) has been used to separate egg-white proteins without cell debris remover (CDR) pretreatment. Results showed that the productivity increased with the superficial velocity (Vs) and a very high Vs (9.2 m/h) can benefit the high productivity (87.4 g/l.h) in comparison with 6.4 and 33 g/l.h as reported by others. Despite the high flow-rate and high column loading, the resolution of the eluted peaks of lysozyme and conalbumin, was maintained at 1.16.

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Authors and Affiliations

  1. School of Chemical Engineering, University of Bath, Bath, UK

    Fang Ming & John A. Howell

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  1. Fang Ming
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  2. John A. Howell
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Ming, F., Howell, J.A. Application of a novel PRODUCTIVTM CM column in egg-white protein separation: Effect of high flow-rate on resolution and productivity. Biotechnol Tech 6, 433–438 (1992). https://doi.org/10.1007/BF02447484

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  • DOI: https://doi.org/10.1007/BF02447484

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