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The effect of temperature on the kinetics of a reverse micellar protein extraction process

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Biotechnology Techniques

Summary

The kinetics of transfer of chymotrypsin from an aqueous into a reverse micellar phase and vice versa has been examined for a two-phase system with planar interface equipped with vibrating disks for separate mixing of both phases. The temperature dependence of the mass-transfer coefficients gives two values for the forward transfer (30 and 90 kJ/mol) depending on the temperature range. Only one value is obtained for the backward transfer (60kJ/mol).

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Abbreviations

K:

Partition coefficient [-]

kFE :

Forward mass-transfer coefficient [m/s]

kBE :

Backward mass-transfer coefficient [m/s]

[CT]org,i :

Organic interfacial α-chymotrypsin concentration [mol]

[CT]aq,i :

Aqueous interfacial α-chymotrypsin concentration [mol]

[CT]org :

Organic bulk α-chymotrypsin concentration [mol]

[CT]aqu :

Aqueous bulk α-chymotrypsin concentration [mol]

JCT :

Initial flux [mol/m2-s]

A:

Interfacial area [m2]

V:

Phase volume [m3]

References

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Authors and Affiliations

  1. Institut de génie chimique, Ecole Polytechnique Fédérale, CH-1015, Lausanne

    M. Hentsch, P. Menoud & A. Renken

  2. Technische Fakultät der Universität, D-4800, Bielefeld

    E. Flaschel

Authors
  1. M. Hentsch
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  2. P. Menoud
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  3. E. Flaschel
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  4. A. Renken
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Hentsch, M., Menoud, P., Flaschel, E. et al. The effect of temperature on the kinetics of a reverse micellar protein extraction process. Biotechnol Tech 6, 423–428 (1992). https://doi.org/10.1007/BF02447482

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  • DOI: https://doi.org/10.1007/BF02447482

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