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Improved thin-layer chromatography separation of carbohydrates in wort and beer

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Biotechnology Techniques

Summary

With a slight modification the method previously reported by Vrbaški and Lepojević,J. Chromatog. 558, 328–332 (1991) can be applied to the analysis of carbohydrates in worts, beer and brewing syrup. The modification introduces new a third step to the development of plates using chloroform/glac. acetic acid/water (2;6;2 by vol). By this method it is possible to separate maltooligosaccharides up to 17 spots. Evidence of a difference between the yeast strains in fermentation of carbohydrate is also presented.

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Vrbaški, L., Markov, S., Jakovljević, J. et al. Improved thin-layer chromatography separation of carbohydrates in wort and beer. Biotechnol Tech 6, 413–416 (1992). https://doi.org/10.1007/BF02447480

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  • DOI: https://doi.org/10.1007/BF02447480

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