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Control of dissolved oxygen concentration in mammalian cell culture using a computer-coupled mass flow controller

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Biotechnology Techniques

Summary

A simple proportional control system for dissolved oxygen (DO) concentration in cell culture medium was developed by using a computer-coupled mass flow controller. The DO levels were very stable during the cultivation of Vero-6, while flow rates of air and/or oxygen enriched air were gradually changed depending on the DO concentration and the preset DO level. Vero-6 cells could grow normally to the confluence in the range of 30% and 50% of DO. Growth of Vero-6 at 10% of DO was markedly retarded.

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Authors and Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science and Technology, 371-1 Kusongdong, Yusung-Gu, 305-701, Taejon, Korea

    Heung C. Jung, Deok K. Oh & Jung H. Kim

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  1. Heung C. Jung
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  2. Deok K. Oh
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  3. Jung H. Kim
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Jung, H.C., Oh, D.K. & Kim, J.H. Control of dissolved oxygen concentration in mammalian cell culture using a computer-coupled mass flow controller. Biotechnol Tech 6, 405–408 (1992). https://doi.org/10.1007/BF02447478

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  • DOI: https://doi.org/10.1007/BF02447478

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