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Characterization of microbial membranes used for the estimation of biochemical oxygen demand with a biosensor

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Biotechnology Techniques

Summary

A bacterial mixed culture was immobilized in Millipore filters to construct microbial-membranes for BOD determination using an oxygen electrode. The biosensor response was best when 0.5 mg cells were immobilized per cm2 of membrane, at 30°C, pH 7 and 0.05 M phosphate buffer. Reproducible microbial-membranes can be constructed and they can be stored for up to 20 days without appreciable loss of their response characteristics.

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References

  1. Galindo, E., Bautista, D., Pimentel, J., Macias, A. Díaz-Nava, P. and Quintero R., (1983) “Application of microbial electrodes to food industry.Prog. in Food Eng. (C. Cantarelli and C. Peri. Ed), Foster-Verlag, Switzerland, p. 409–412.

    Google Scholar 

  2. Galindo, E. and Quintero, R., (1983), “Electrodo microbiano para la determinación de la DBO”, in Biotecnologia de enzimas, Ed. C. Huitrón, UNAM, México, 364–368.

    Google Scholar 

  3. Harita, K., Otani, Y., Hikuma, M. and Yasuda, T., (1985), “BOD quick estimating system utilizing microbial electrode”,Instrumental Control. Water, Waste Water Treatment Transport System. Proc IAWPRC Workshop,4: 529–532.

    Google Scholar 

  4. Hikuma, M., Suzuki, H., Yasuda, T., Karube, I. and Suzuki, S., (1979), “Amperometric estimation of BOD by using living immobilized yeast”,European J. Appl. Microbiol., 8: 289–297.

    Article  CAS  Google Scholar 

  5. Karube, I., Matsunaga, T., Mitsuda, S. and Suzuki, S., (1977a), “Microbial electrode BOD sensors”,Biotechnol. Bioeng. 19: 1535–1547.

    Article  CAS  Google Scholar 

  6. Karube, I., Mitsuda, S., Matsunaga, T. and Susuki, S., (1977b). “A rapid method for estimation of BOD by using immobilized microbial cells”,J. Ferment. Technol. 55(3): 243–248.

    CAS  Google Scholar 

  7. Karube, I., Matsunaga, T. and Susuki, S., (1977c), “A new microbial electrode for BOD estimation”,J. solid Phase Biochem. 2(2): 97–104.

    CAS  Google Scholar 

  8. Karube, I., (1990), “Microbial sensor”,J. Biotechnol.,15, 255–256.

    Article  CAS  Google Scholar 

  9. Kulys, J., Kadziauskiené, K., (1980), “Yeast BOD sensor”,Biotechnol. Bioeng. 22: 221–226.

    Article  CAS  Google Scholar 

  10. Public Health Association (1971), Standard methods for examination of water and waste water, 474–478, Yearbook Pub., Chicago, Ill.

    Google Scholar 

  11. Riedel, K., Renneberg, M. and Scheller., (1988), “A fast estimation of biochemical oxygen demand using microbial sensors”,Appl. Microbiol. Biotechnol. 28: 316–318.

    CAS  Google Scholar 

  12. Strand, S. E. and Carlson, D. A., (1984), “Rapid BOD measurement for municipal waste-water samples using a biofilm electrode”,J. Water Pollut Control Fed. 56: 464–467.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Departmento de Bioingeniería, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, 62271, Cuernavaca, Mor., México

    E. Galindo, J. L. García, L. G. Torres & R. Quintero

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  1. E. Galindo
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  2. J. L. García
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  3. L. G. Torres
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  4. R. Quintero
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Galindo, E., García, J.L., Torres, L.G. et al. Characterization of microbial membranes used for the estimation of biochemical oxygen demand with a biosensor. Biotechnol Tech 6, 399–404 (1992). https://doi.org/10.1007/BF02447477

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  • DOI: https://doi.org/10.1007/BF02447477

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