Summary
A simple method using non-linear regression is developed to analyse experimental data from plasmid stability studies of recombinantSaccharomyces cerevisiae grown in continuous cultures with non-selective and selective media. This method simultaneously provides quantitative information on the probability of plasmid loss due to segregation during cell division and the specific growth rates of plasmid-containing and plasmid-free cells at particular dilution rates. The method is applied to a set of experimental data. The three-parameter model, together with the estimated parameter values, provides a good fit to the experimental data.
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Abbreviations
- N1:
-
Number of plasmid-containing cells per unit volume at time t
- N2:
-
Number of plasmid-free cells per unit volume at time t
- NT:
-
Total number of cells per unit volume at time t
- p:
-
Probability of plasmid loss
- t:
-
Time
- D:
-
Dilution rate
- OD:
-
Optical density
- α:
-
Fraction of plasmid-containing cells
- μ1:
-
Specific growth rate of plasmid-containing cells
- μ2:
-
Specific growth rate of plasmid-free cells
- β-GAL:
-
β-galactosidase expression
- o:
-
Initial values
References
Caunt, P., Impoolsup, A. and Greenfield, P.F. (1988) J. Biotechnol. 8, 173–192
Cooper, N.S., Brown, M.E., Caulcott, C.A. (1987). J. Gen. Microbiol., 133, 1871–1880
Futcher, A.B., and Cox, B.S. (1983). J. Bacteriol, 154, 612–622
Hartwell, L.H. and Unger, M. (1977). J. Cell. Biol. 75, 422–435
Helling, R.B., Kinney, T., Adams, J. (1981). J. Gen. Microbiol, 123, 129–141
Imanaka, T and Aiba, S. (1981) Ann. N. Y. Acad. Sci., 369, 1–14
Impoolsup, A. Caunt, P., Greenfield, P. F. (1989). J. Biotechnol. 10, 171–180
Impoolsup, A. (1990). PhD Thesis. University of Queensland.
Jones, S. A., Primrose, S. B., Robinson, A. and Elwood, D. C. (1980). Mol. Gen. Genet., 180, 579–584
Lord, P. G. and Wheals, A. E. (1980). J. Bacteriol., 142, 808–818
Mead, D. J., Gardner, D.C.J., and Oliver, S. G. (1986). Mol. Gen. Genet. 205, 417–421
Noack, D., Roth, M., Geuther, R, Muller, G. and Undisz, K. (1980). Mol. Gen. Genet., 184, 121–124
Sardonini, C. A. and DiBiasio, D. (1987). Biotechnol. Bioeng. 29, 469–475
Srienc, F., Campbell, J. L., and Bailey, J. E. (1986). Biotechnol. Bioeng., 28, 996–1006
San, K.Y., and Weber, A.E. (1989). Biotechnol. Bioeng., 33, 451–459
Zabriskie, D. and Arcuri, E. J. (1986). Enz. Microb. Technol., 8, 706–717
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Syamsu, K., Impoolsup, A., Greenfield, P.F. et al. Data analysis of plasmid stability in continuous culture of recombinantSaccharomyces cerevisiae . Biotechnol Tech 6, 393–398 (1992). https://doi.org/10.1007/BF02447476
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DOI: https://doi.org/10.1007/BF02447476