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Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes

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Abstract

Transmembrane potentials on the plasma (Δφ4) and mitochondrial (Δφm) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were −93±4 and −196±11 mV, respectively. Sufan significantly decreased the reduction of Δφm induced by chemical hypoxia. The effects of antiarrhythmic drugs on changes in Δφp were studied using the fluorescent probe dis-C3-(5). Lidocaine, novocainamide, richlocaine, and leocaine blocked depolarization of the myocyte plasma membrane induced by electrical stimulation and did not affect the Δφm.

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Authors and Affiliations

  1. Department of Pharmacology, Kuban Medical Academy, Krasnodar

    A. I. Khankoeva, A. S. Dukhanin & P. A. Galenko-Yaroshevskii

  2. Department of Molecular Pharmacology with a Radiobiology Course, Russian State Medical University, Moscow

    A. I. Khankoeva, A. S. Dukhanin & P. A. Galenko-Yaroshevskii

Authors
  1. A. I. Khankoeva
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  2. A. S. Dukhanin
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  3. P. A. Galenko-Yaroshevskii
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 11, pp. 594–597, November, 1998

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Khankoeva, A.I., Dukhanin, A.S. & Galenko-Yaroshevskii, P.A. Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes. Bull Exp Biol Med 126, 1170–1172 (1998). https://doi.org/10.1007/BF02447265

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  • DOI: https://doi.org/10.1007/BF02447265

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