Bulletin of Experimental Biology and Medicine

, Volume 122, Issue 4, pp 1044–1046 | Cite as

Saponin in thein vitro phosphorylation system

  • S. N. Echikov
  • T. G. Shchipakina
  • V. I. Arkhipov
New Biomedical Technologies

Abstract

The possibility of the use of saponin for permeabilization of synaptic vesicles from P2 fraction is explored. This fraction is isolated from rat brain cortex. Phosphorylation of membrane-bound proteins is performedin vitro. At the saponin:protein ratio 1:10 (w/w) phosphorylation of these proteins increases 2-fold due to increased membrane permeability for ATP and cAMP. Treatment of synaptic vesicles with saponin at the same ratio leads to the loss of cytosolic but not membrane-bound protein. This concentration of saponin can be used for permeabilization of other membranes, providing that the protein:cholesterol ratio is taken into consideration.

Key Words

saponin protein phosphorylation synaptic membranes synaptosomes 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • S. N. Echikov
    • 1
  • T. G. Shchipakina
    • 1
  • V. I. Arkhipov
    • 1
  1. 1.Research Group for the Investigation of Synaptic Transmission, Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchino

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