A study of the mechanism of action of befol on Ca2+ metabolism in cardiomyocytes using a FURA-2 fluorescent probe
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The dynamics of the Ca-response of cardiomyocytes is studied and the efficiency of befol, verapamil, and amiodarone is compared using various experimental models of stimulation of [Ca2+]i. Befol (1–5 μM) is shown to inhibit the caffeine-and strophanthin G-induced rise of [Ca2+]i. Unlike verapamil and amiodarone, befol exhibits no Ca-blocking activity in modeled K-depolarization. It is concluded that the cardiotropic effect of befol is mediated through its primary action on Na+/Ca2+ exchange in cardiomyocytes, while the cardioplegic effect of verapamil and amiodarone is due to their ability to block the slow Ca2+ inward current.
Key Wordscalcium befol amiloride strophanthin caffeine cardiomyocytes
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