Abstract
The use of monoclonal antibodies against Taq DNA polymerase in the polymerase chain reaction is proposed. These antibodies effectively inhibit polymerase activity at temperatures <70°C, activity being restored upon DNA melting in the very first stage of amplification. As in the “hot start” reaction, the addition of the antibodies to the incubation medium markedly improves the reaction yield. The method is particularly effective for the identification of a few copies of DNA.
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Q. Chou, M. Russell, D. Birch,et al., Nucleic Acids Res.,20, 1717–1723 (1992).
R. T. D'Aquila, L. J. Bechtel, J. A. Videler,et al., —Ibid.,,19, 3749 (1991).
R. M. Horton, B. L. Hoppe, and B. M. Conti-Tronconi,Bio-Techniques,16, 42–43 (1994).
S. Kwok and R. Higuchi,Nature,339, 237–238 (1989).
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, No 2, pp. 238–240, February, 1996
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Rybalkin, I.N., Mukhamedova, N.M., Vlasik, T.N. et al. The new technology of hot start polymerase chain reaction. Bull Exp Biol Med 121, 221–223 (1996). https://doi.org/10.1007/BF02446641
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DOI: https://doi.org/10.1007/BF02446641