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Determination of fructose 2,6-bisphosphate in human lymphocytes is a potential laboratory test in diabetology

  • Experimental Methods for Clinical Practice
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Abstract

The conventional micromethod of fructose 2,6-bisphosphate (F-2,6-P2) determination is optimized for human peripheral blood lymphocytes. The F-2,6-P2 content in lymphocytes of healthy subjects is 0.8±0.2 pmol/106 cells or 6.7±1.1 pmol/mg protein. Preliminary findings show a decrease in the F-2,6-P2 content in lymphocytes from patients with severe diabetes mellitus.

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References

  1. T. Yu. Budennaya, N. F. Belyaeva, and B. F. Korovkin,Lab. Delo, No. 7, 22–25 (1990).

    Google Scholar 

  2. B. F. Korovkin, N. F. Belyaeva, M. A. Golubev,et al., Klin. Lab. Diagn., No. 2, 21–24 (1994).

    PubMed  Google Scholar 

  3. Lymphpocytes. Methods [in Russian], Moscow (1990), pp. 60–63.

  4. Farmatsia. Production Catalogue [in Russian] (1983), p. 73.

  5. L. Bosca, M. Mogena J. M. Giar-Guerra, and C. Marques,Eur. J. Biochem.,175, 317–323 (1988).

    Article  PubMed  CAS  Google Scholar 

  6. M. A. Bradford,Anal. Biochem.,72, 248–254 (1976).

    Article  PubMed  CAS  Google Scholar 

  7. P. Bruni, V. Vasta, and M. Farnararo,,178, No. 2, 324–326 (1989).

    Article  PubMed  CAS  Google Scholar 

  8. D. Colomer, J. L. Vives Corrons, and R. Bartrons,Biochim. Biophys. Acta,1097, 270–274 (1991).

    PubMed  CAS  Google Scholar 

  9. D. Colomer, J. L. Vives Corrons, A. Pujades, and R. Bartrons,Cancer Res.,47, 1859–1862 (1987).

    PubMed  CAS  Google Scholar 

  10. M. A. Golubev, N. S. Stvolinskaya, M. S. Markovaet al., Pathobiochemical Aspects of Extreme States, Moscow, 68–79 (1993).

  11. J. H. Helderman,J. Clin. Invest.,67, 1636–1642 (1981).

    Article  PubMed  CAS  Google Scholar 

  12. L. Hue and M. H. Rider,Biochem. J.,245, 313–324 (1987).

    PubMed  CAS  Google Scholar 

  13. C. Murano, Y. Inoue, M. Emoto,et al., Eur. J. Pharmacol.,254, 257–262 (1994).

    Article  PubMed  CAS  Google Scholar 

  14. K. Y. Tu, F. Pettit, W. Shive,et al., Biochem. Biophys. Res. Commun.,207, No. 1, 183–190 (1995).

    Article  PubMed  CAS  Google Scholar 

  15. E. Van Schaftingen, B. Lederer, R. Bartrons, and H.-G. Hers,Eur. J. Biochem.,129, 191–195 (1982).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Enzymology of Extreme States, Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow

    N. F. Belyaeva, M. A. Golubev, M. S. Markova, O. L. Kol'chenko, N. N. Lamzina, V. K. Gorodetskii, L. N. Viktorova, M. I. Balabolkin & B. F. Korovkin

  2. Institute of Diabetes, Endocrinology Research Center, Russian Academy of Medical Sciences, Moscow

    N. F. Belyaeva, M. A. Golubev, M. S. Markova, O. L. Kol'chenko, N. N. Lamzina, V. K. Gorodetskii, L. N. Viktorova, M. I. Balabolkin & B. F. Korovkin

Authors
  1. N. F. Belyaeva
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  2. M. A. Golubev
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  3. M. S. Markova
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  4. O. L. Kol'chenko
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  5. N. N. Lamzina
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  6. V. K. Gorodetskii
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  7. L. N. Viktorova
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  8. M. I. Balabolkin
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  9. B. F. Korovkin
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Additional information

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 9, pp. 341–344, September, 1996

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Belyaeva, N.F., Golubev, M.A., Markova, M.S. et al. Determination of fructose 2,6-bisphosphate in human lymphocytes is a potential laboratory test in diabetology. Bull Exp Biol Med 122, 950–952 (1996). https://doi.org/10.1007/BF02446591

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  • DOI: https://doi.org/10.1007/BF02446591

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