Bulletin of Experimental Biology and Medicine

, Volume 120, Issue 2, pp 763–766 | Cite as

Inverter mechanism of the influence exerted by insulin on the plasma membranes of cardiac myocytes in rats of different age

  • V. V. Frol'kis
  • O. K. Kul'chitskii
  • E. N. Gorban'


In the plasma membranes of cardiac myocytes from old rats, Na, K-ATPase activity and phosphatidylinositol levels were lower and cardiolipin levels higher than in those from younger (adult) animals. Insulin injected into adult rats elevated Na, K-ATPase activity and phosphatidylethanolarnine levels and caused a sharp fall in phosphatidylinositol levels. In old rats, insulin had no effect on Na, K-ATPase activity, but lowered phosphatidylethanolamine levels. In experiments with cellular hybrids (cytosol+plasma membranes), cytosol from adult rats activated Na, K-ATPase in both adult and old rats, whereas cytosol from old rats failed to activate the enzyme both in old and in adult rats. Actinomycin D prevented the stimulatory effect of insulin on Na,K-ATPase activity.

Key Words

insulin myocardium membranes aging 


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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • V. V. Frol'kis
    • 1
  • O. K. Kul'chitskii
    • 1
  • E. N. Gorban'
    • 1
  1. 1.Laboratory of Physiology and Laboratory of Metabolic Regulation, Institute of GerontologyUkrainian Academy of Medical SciencesKiev

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