Abstract
Intravitam phase-contrast microscopy of individual isolated myelin fibers of rabbit tibial nerves shows that 40-min treatment with 0.2% pronase solution is harmless to intact myelin fibers. In injured nerves such treatment causes sharply expressed proteolysis and a rapid process of wallerian degeneration with ruptures of the axial cylinders and the formation of degeneration ovoids. The findings prove the feasibility and desirability of early primary protease treatment of nerve wounds.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
O. S. Sotnikov,Functional Morphology of a Living Medullary Nerve Fiber [in Russian], Leningrad (1976).
A. P. Khokhlov and Yu. N. Savchenko,Myelinopathy and Demyelinating Diseases [in Russian], Moscow (1990).
E. I. Chumasov, G. N. Akoev, L. I. Kolosova,et al., Arkh. Anat., No 2, 6–13 (1988).
N. Azzam, A. Zalewski, L. Williams,et al., J. Comp. Neurol.,314, 807–819 (1991).
B. Juurlink, D. Munoz, and L. Ang,Neurosci. Lett.,133, 25–28 (1991).
S. Rochkind and G. Quaknine,Neurol. Res.,14, 2–11 (1992).
N. Suematsu,Microsurgery,10, 72–74 (1989).
A. Warschell, G. Naray-Szabo, F. Sussman,et al., Biochemistry,28, 3629–3637 (1989).
Author information
Authors and Affiliations
Additional information
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, No 4, pp. 439–442, April, 1995
Rights and permissions
About this article
Cite this article
Sotnikov, O.S., Yastrembski, M., Insezharova, G.M. et al. Morphological analysis of protease treatment of damaged nerves. Bull Exp Biol Med 119, 425–428 (1995). https://doi.org/10.1007/BF02445912
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02445912