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Detection of STLV-1 integration in DNA extracted from formalin-fixed and paraffin-embedded specimens ofM. Arctoides lymphomas using the polymerase chain reaction

  • Microbiology and Immunology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The feasibility of using the polymerase chain reaction to analyze of DNA extracted by the conventional method from formalin-fixed and paraffin-embedded specimens of monkey lymphoid tissue was investigated. Using HTLV-1 primers that can amplify DNA fragments of various length (159 to 717 base pairs), we determined the size of formalin-destroyed DNA which appeared to be up to 500 base pairs in length. Analysis of four specimens ofM. arctoides lymphomas that had been stored since 1969 revealed integrated STLV-1 provirus.

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Authors and Affiliations

  1. Institute of Medical Primatology, Sochi-Adlen

    M. G. Chikobava, L. A. Yakovleva, L. V. Indzhiya & B. A. Lapin

  2. M. Pettenkofer Institute of Medical Microbiology and Hygiene, Munich

    G. Schatzel

Authors
  1. M. G. Chikobava
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  2. L. A. Yakovleva
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  3. L. V. Indzhiya
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  4. G. Schatzel
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  5. B. A. Lapin
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, No 3, pp. 314–316, March, 1995

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Chikobava, M.G., Yakovleva, L.A., Indzhiya, L.V. et al. Detection of STLV-1 integration in DNA extracted from formalin-fixed and paraffin-embedded specimens ofM. Arctoides lymphomas using the polymerase chain reaction. Bull Exp Biol Med 119, 306–308 (1995). https://doi.org/10.1007/BF02445844

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  • DOI: https://doi.org/10.1007/BF02445844

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