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Assessment of the toxic and protective effects of initiators and inhibitors of free radical reactions using a wild-type strain ofEscherichia coli and a strain deficient for superoxide dismutase

  • Microbiology and Immunology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The toxic effects of H2O2, paraquat, and oxidized low density lipoproteins are more expressed on superoxide dismutase-deficientE. coli strains than on its wild-type strains, and the effect of tert-butyl-hydroperoxide is less dependent on the presence or absence of this enzyme in the bacterium, whereas that of bleomycin does not depend on it at all. The toxicity of the agents increases in the following series: H2O2<oxidized low density lipoproteins<tert-butyl-hydroperoxide <paraquat≪bleomycin. A culture ofE. coli strains AB 1157 and JI 132 may be used for assessing the toxic effect of prooxidants, and anE. coli JI 132 culture with oxidative stress induced by prooxidants as a test system for detecting the potential antioxidants and assessing the mechanism of the action.

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Authors and Affiliations

  1. Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow

    M. V. Bilenko, V. G. Ladygina & I. A. Tarakanova

Authors
  1. M. V. Bilenko
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  2. V. G. Ladygina
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  3. I. A. Tarakanova
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, No, pp. 74–79, January, 1996

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Bilenko, M.V., Ladygina, V.G. & Tarakanova, I.A. Assessment of the toxic and protective effects of initiators and inhibitors of free radical reactions using a wild-type strain ofEscherichia coli and a strain deficient for superoxide dismutase. Bull Exp Biol Med 121, 67–71 (1996). https://doi.org/10.1007/BF02445710

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  • DOI: https://doi.org/10.1007/BF02445710

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