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Bulletin of Experimental Biology and Medicine

, Volume 120, Issue 6, pp 1221–1224 | Cite as

Identification ofMycoplasma hominis, Ureaplasma urealyticum, andChlamydia trachomatis by the polymerase chain reaction

  • M. Yu. Brodskii
  • V. M. Govorun
  • E. M. Khalilov
  • A. V. Zhdanov
  • L. Z. Faizullin
  • G. T. Sukhikh
Microbiology and Immunology
  • 25 Downloads

Abstract

A procedure for identifying chlamydia, mycoplasmas, and ureaplasmas by the polymerase chain reaction is described which uses a system of four primers complementary to fragments of the 16S RNA gene, one of which detects all three microorganisms and the other three are each specific for a particular organism. With this primer system, chlamydia, mycoplasmas, and ureaplasmas are detectable in a single reaction. The procedure may be used for routine diagnoses in diagnostic laboratories.

Key Words

polymerase chain reaction Mycoplasma hominis Ureaplasma urealyticum Chlamydia trachomatis 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • M. Yu. Brodskii
    • 1
  • V. M. Govorun
    • 1
  • E. M. Khalilov
    • 1
  • A. V. Zhdanov
    • 2
  • L. Z. Faizullin
    • 2
  • G. T. Sukhikh
    • 2
  1. 1.Institute of Physicochemical MedicineMinistry of Health and Medical Industry of the Russian FederationMoscow
  2. 2.Center of Obstetrics, Gynecology, and PerinatologyRussian Academy of Medical SciencesMoscow

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