Bulletin of Experimental Biology and Medicine

, Volume 120, Issue 5, pp 1157–1161 | Cite as

Morphological changes in red cells exposed to perfluorodecalin and oxygenation

  • V. I. Skorik
  • A. P. Novozhilova
  • A. V. Sudus
  • V. V. Shilov
Morphology and Pathomorphology


The hemoprotective effect of perfluorocarbons was studied by examining the surface architectonics of red cells during exposure of the blood to perfluorodecalin and contact oxygenation. Scanning electron microscopy revealed an, appeciable increase in the percentage of damaged erythrocytes directly exposed to oxygen in a contact oxygenator. Interaction between blood and perfluorodecalin not only does not impair the morphology of the blood, but also prevents untoward effects of subsequent contact oxygenation. Fluorocarbon treatment of the blood gives rise to a number (up to 17–18%) of special cells, which morphologically represent somewhat altered normocytes with increased resistance, this reflecting a specific reaction of the blood to perfluorodecalin.

Key words

perfluorodecalin blood oxygenation electron microscopy morphological changes 


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  1. 1.
    F. F. Beloyartsev, in:Perfluorinated Carbons in Biology and Medicine [in Russian], Pushchino (1980), pp. 5–21.Google Scholar
  2. 2.
    R. V. Bobrovskii, V. A. Buevich, V. V. Moroz,et al., in:Fluorocarbon Gas-Transporting Media [in Russian], Pushchino (1984), pp. 27–32.Google Scholar
  3. 3.
    G. D. Ioseliani, S. M. Chilaya, and f. F. Beloyartsev,Byull. Izobret., № 10 (1986).Google Scholar
  4. 4.
    V. S. Kasulin, Kh. Kh. Khapii, F. F. Beloyartsev,et al., Ibid., Byull. Izobret., № 36 (1980).Google Scholar
  5. 5.
    L. D. Krymskii, G. V. Nestaiko and A. G. Rybalov, in:Scanning Electron Microscopy of Blood Vessels and Blood [in Russian], Moscow (1976), pp. 37–48.Google Scholar
  6. 6.
    V. V. Obraztsov and Yu. S. Tarakhovskii, in:Fluorocarbon Gas-Transporting Media [in Russian], Pushchino (1984), pp. 156–162.Google Scholar
  7. 7.
    T. A. Ostrovskaya, N. V. Ermolenko, V. I. Fedenkov,et al., in:Perfluorinated Carbons in Biology and Medicine, [in Russian], Pushchino (1980), pp. 90–93.Google Scholar
  8. 8.
    V. I. Skorik, I. Kh. Gershengorn, S. P. Kazakov,et al., A Blood Oxygenator, Pat. № 5036598/14 of July 14, 1991.Google Scholar
  9. 9.
    V. I. Skorik, B. M. Zelikson, D. D. Cherkas,et al., Byull. Izobret., № 45 (1991).Google Scholar
  10. 10.
    E. A. Chernitskii and A. V. Vorobei, in:Structure and Function of Erythrocyte Membranes [in Russian], Minsk (1981), pp. 92–111.Google Scholar
  11. 11.
    S. M. Chilaya, D. A. Dvaladze, A. G. Gogoshvili,et al., Anest. Reanimatol., № 5, 36–40 (1985).Google Scholar
  12. 12.
    H. W. Wallace, W. J. Asher, and N. N. Li,Trans. Amer. Soc. Artif. Intern. Organs,19, 80–86 (1973).Google Scholar
  13. 13.
    H. W. Wallace, T. P. Stein, and W. J. Asher,Fed. Proc.,34, № 6, 1506–1509 (1975).PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • V. I. Skorik
    • 1
  • A. P. Novozhilova
    • 1
  • A. V. Sudus
    • 1
  • V. V. Shilov
    • 1
  1. 1.P. A. Kupriyanov Clinic for Cardiovascular SurgeryMilitary Medical AcademySt. Petersburg

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