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Bulletin of Experimental Biology and Medicine

, Volume 124, Issue 6, pp 1203–1205 | Cite as

Effect of oxidized cholesterol derivatives on lymphokine-stimulated macrophage differentiation and primary allogenic mixed culture of human lymphocytes

  • M. I. Musatov
  • M. I. Dushkin
  • N. N. Vol'skii
  • O. M. Perminova
  • V. I. Konenkov
  • V. A. Kozlov
Microbiology and Immunology

Abstract

Products of cholesterol oxidation, 25-hydroxycholesterol and 7-ketocholesterol, in a dose of 1 μg/ml considerably suppress proliferative response in a primary one-way mixed culture of human lymphocytes and have no effect on spontaneous proliferation. Preliminary 18-h separate culturing of the stimulator and responders with these lipids shows that inhibiting effect of these lipids on cell proliferation is associated with their influence on both stimulator and, to a greater extent, responders. Both lipids in the same dose inhibit accumulation of HLA-DR-positive macrophages in a 4-day culture of peripheral blood adherent cells stimulated with lymphokine-containing supernatant.

Key Words

oxidized cholesterol macrophages HLA-DR expression mixed lymphocyte culture 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • M. I. Musatov
    • 1
    • 2
  • M. I. Dushkin
    • 1
    • 2
  • N. N. Vol'skii
    • 1
    • 2
  • O. M. Perminova
    • 1
    • 2
  • V. I. Konenkov
    • 1
    • 2
  • V. A. Kozlov
    • 1
    • 2
  1. 1.Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical SciencesNovosibirsk
  2. 2.Institute of TherapySiberian Division of the Russian Academy of Medical SciencesNovosibirsk

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