Effect of oxidized cholesterol derivatives on lymphokine-stimulated macrophage differentiation and primary allogenic mixed culture of human lymphocytes
Products of cholesterol oxidation, 25-hydroxycholesterol and 7-ketocholesterol, in a dose of 1 μg/ml considerably suppress proliferative response in a primary one-way mixed culture of human lymphocytes and have no effect on spontaneous proliferation. Preliminary 18-h separate culturing of the stimulator and responders with these lipids shows that inhibiting effect of these lipids on cell proliferation is associated with their influence on both stimulator and, to a greater extent, responders. Both lipids in the same dose inhibit accumulation of HLA-DR-positive macrophages in a 4-day culture of peripheral blood adherent cells stimulated with lymphokine-containing supernatant.
Key Wordsoxidized cholesterol macrophages HLA-DR expression mixed lymphocyte culture
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