Bulletin of Experimental Biology and Medicine

, Volume 124, Issue 1, pp 668–671 | Cite as

λ-Radiation-induced irreparable damage to DNA of HeLa cells

  • V. A. Struchkov
  • N. I. Demidova
  • N. B. Strazhevskaya
Biophysics and Biochemistry
  • 29 Downloads

Abstract

Exposure of HeLa cells to λ-radiation at 0.1 Gy and then at 5 Gy reduces their ability to repair double-strand DNA breaks to a greater extent than irradiation with a single dose of 5 Gy. Modifying effects of 0.1 Gy on double-strand DNA breaks and on cell survival are observed after irradiation during logarithmic but not stationary phase of growth. Primary λ-induced irreparable double-strand breaks correlates with cell survival regardless the irradiation regime. It is suggested that such a damage is primarily responsible for reproductive death of HeLa cells.

Key Words

λ-radiation DNA damage HeLa cells 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • V. A. Struchkov
    • 1
  • N. I. Demidova
    • 1
  • N. B. Strazhevskaya
    • 1
  1. 1.Laboratory of Cellular Metabolism, Institute of Experimental Diagnosis and Therapy of Tumors, Oncology Research CenterRussian Academy of Medical SciencesMoscow

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