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Detection of antibodies to HLA1 antigens by enzyme immunoassay

  • Experimental Methods for Clinical Practice
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A modification of the MAILA (monoclonal antibody specific immobilization of lymphocyte antigens) method has been developed for the detection of antibodies to class 1 histocompatibility antigens. Russian biotin-treated monoclonal antibodies IKO-53 (Medbiospektr, Moscow) were used. In a complex with monoclonal antibodies, lymphocyte HLA antigen was found to retain its antigenic properties when stored for a long time. High specificity and sensitivity of the method were demonstrated.

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Authors and Affiliations

  1. Department of Immunology, Research Institute of Transplantology and Artificial Organs, Ministry of Health of Russia, Moscow

    S. D. Borozdenkova, A. Yu. Baryshnikov, T. N. Palkina & F. S. Baranova

  2. Department of Experimental Diagnosis of Tumors, Cancer Research Center, Russian Academy of Medical Sciences, Moscow

    S. D. Borozdenkova, A. Yu. Baryshnikov, T. N. Palkina & F. S. Baranova

Authors
  1. S. D. Borozdenkova
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  2. A. Yu. Baryshnikov
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  3. T. N. Palkina
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  4. F. S. Baranova
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, No 9, pp. 315–317, September, 1995

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Borozdenkova, S.D., Baryshnikov, A.Y., Palkina, T.N. et al. Detection of antibodies to HLA1 antigens by enzyme immunoassay. Bull Exp Biol Med 120, 954–956 (1995). https://doi.org/10.1007/BF02445028

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  • DOI: https://doi.org/10.1007/BF02445028

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