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Enzyme immunoassay of lipoprotein(a)

  • Microbiology and Immunology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Sheep polyclonal antibodies to human lipoprotein(a) were used for the development of sandwich enzyme immunoassay. These antibodies virtually do not interact with human plasminogen, apoB-100, or fibrinogen in this assay. The method permits measurements of lipoprotein(a) in a wide range of concentrations: from 2 to 500 mg/dl. The variability of analyses within the range of 5 to 180 mg/dl is approximately 4.5% in a plate and 10.5% in different tests. Correlation analysis of the results of the enzyme immunoassay modification developed in our laboratory and of its foreign analogs showed a high similarity of the methods, the correlation coefficient being 95%.

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Authors and Affiliations

  1. Research Institute of Experimental Cardiology, Cardiology Research Center, Russian Academy of Medical Sciences, Moscow

    O. I. Afanas'eva, I. Yu. Adamova, G. F. Benevolenskaya & S. N. Pokrovskii

Authors
  1. O. I. Afanas'eva
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  2. I. Yu. Adamova
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  3. G. F. Benevolenskaya
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  4. S. N. Pokrovskii
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, No 10, pp. 398–401, October, 1995

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Afanas'eva, O.I., Adamova, I.Y., Benevolenskaya, G.F. et al. Enzyme immunoassay of lipoprotein(a). Bull Exp Biol Med 120, 1030–1033 (1995). https://doi.org/10.1007/BF02444976

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  • DOI: https://doi.org/10.1007/BF02444976

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