Bulletin of Experimental Biology and Medicine

, Volume 118, Issue 5, pp 1189–1192 | Cite as

Specificity of monoclonal antibodies obtained by immunization of mice with trypsin-treated group a streptococcus culture

  • T. A. Danilova
  • T. K. Asoskova
  • N. A. Borodiyuk
  • L. V. Beletskaya
  • V. G. Nesterenko
Microbiology and Immunology


Hybridomas producing monoclonal antibodies intensively reacting with group A streptococcus antigens in enzyme immunoassay were obtained as a result of immunizing mice with pepsin-treated cultures of group A streptococcus. All antibodies were referred to class M immunoglobulins. The reactions of monoclonal antibodies were completely inhibited by the pepsin-treated culture of group A streptococcus. The degree of inhibition with A-polysaccharide was lower, being 17.5 to 50.0 in different monoclonal antibodies. All the monoclonal antibodies obtained cross-reacted with antigens of murine and human epithelial tissues of the thymus and skin.

Key Words

streptococcus A-polysaccharide monoclonal antibodies cross-reacting antigens 


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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • T. A. Danilova
    • 1
  • T. K. Asoskova
    • 1
  • N. A. Borodiyuk
    • 1
  • L. V. Beletskaya
    • 1
  • V. G. Nesterenko
    • 1
  1. 1.N. F. Gamaleya Research Institute of Epidemiology and MicrobiologyRussian Academy of Medical SciencesMoscow

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