Bulletin of Experimental Biology and Medicine

, Volume 118, Issue 1, pp 761–763 | Cite as

Dot hybridization and polymerase amplification of DNA in the prenatal diagnosis of cytomegalovirus infection

  • S. A. Sel'kov
  • G. N. Vedeneeva
  • M. V. Aseev
Microbiology and Immunology
  • 19 Downloads

Abstract

Molecular biology techniques (dot hybridization and polymerase amplification of DNA) are used to diagnose cytomegalovirus infection in pregnant women and newborns. It is concluded from the results that dot hybridization is a convenient tool in screening studies and that polymerase DNA amplification followed by verification of the reaction product using restriction enzymes and blot hybridization makes the diagnosis of this disease more reliable and may find wide application in practical laboratories.

Key Words

cytomegalovirus infection prenatal diagnosis dot hybridization polymerase DNA amplification 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • S. A. Sel'kov
    • 1
  • G. N. Vedeneeva
    • 1
  • M. V. Aseev
    • 1
  1. 1.D. O. Otta Research Institute of Obstetrics and GynecologySt. Petersburg

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