Activation of interleukin-2 gene transcription by nuclear factors from the spleen and brain of immunized rats
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The nuclear protein factors which stimulate synthesis of mRNA of interleukin-2 (IL-2) in the system of surviving murine T lymphocytes are isolated from cells of the spleen and brain of immunized rats. The active fractions of spleen and brain (13–19 and 25 kD, respectively), which interact with the promoter-enhancer sequences of the IL-2 gene localized in the region from +39 to −141 base pairs, are isolated by the method of high performance liquid chromatography from the total nuclear extract. The induction of luciferase synthesis is shown for protein factor-treated Jurkat cells transformed with p-IL-2LUC recombinant plasmid.
Key Wordsinterleukin-2 gene protein factors regulation
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