Activation of interleukin-2 gene transcription by nuclear factors from the spleen and brain of immunized rats

  • T. B. Kazakova
  • T. V. Grishina
  • O. I. Golovko
  • G. V. Gushchin
  • A. A. Myul'berg
Experimental Genetics

Abstract

The nuclear protein factors which stimulate synthesis of mRNA of interleukin-2 (IL-2) in the system of surviving murine T lymphocytes are isolated from cells of the spleen and brain of immunized rats. The active fractions of spleen and brain (13–19 and 25 kD, respectively), which interact with the promoter-enhancer sequences of the IL-2 gene localized in the region from +39 to −141 base pairs, are isolated by the method of high performance liquid chromatography from the total nuclear extract. The induction of luciferase synthesis is shown for protein factor-treated Jurkat cells transformed with p-IL-2LUC recombinant plasmid.

Key Words

interleukin-2 gene protein factors regulation 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • T. B. Kazakova
    • 1
    • 2
  • T. V. Grishina
    • 1
    • 2
  • O. I. Golovko
    • 1
    • 2
  • G. V. Gushchin
    • 1
    • 2
  • A. A. Myul'berg
    • 1
    • 2
  1. 1.Research Institute of Experimental MedicineRussian Academy of Medical SciencesUSSR
  2. 2.State UniversitySt. Petersburg

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