Bulletin of Experimental Biology and Medicine

, Volume 117, Issue 3, pp 294–296 | Cite as

Production of bifunctional monoclonal antibodies to human IgG and horseradish peroxidase and their utilization for testing anti-HIV antibodies

  • Yu. S. Massino
  • L. L. Sukhanova
  • E. A. Kizim
  • O. L. Segal
  • M. B. Smirnova
  • V. V. Monakhov
  • G. I. Kolyaskina
  • A. D. Dmitriev
Microbiology and Immunology

Abstract

Bispecific antibodies are purified from ascitic fluid by successive affinity chromatography on HRP-Sepharose and IgG-Sepharose. The ability of the affinity purified bispecific antibodies to bind human IgG and horseradish peroxidase is demonstrated in the immunoenzyme assay (identification of anti-HIV antibodies.

Key Words

quadroma bispecific antibodies human IgG peroxidase anti-HIV antibodies 

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References

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Yu. S. Massino
    • 1
    • 2
    • 3
  • L. L. Sukhanova
    • 1
    • 2
    • 3
  • E. A. Kizim
    • 1
    • 2
    • 3
  • O. L. Segal
    • 1
    • 2
    • 3
  • M. B. Smirnova
    • 1
    • 2
    • 3
  • V. V. Monakhov
    • 1
    • 2
    • 3
  • G. I. Kolyaskina
    • 1
    • 2
    • 3
  • A. D. Dmitriev
    • 1
    • 2
    • 3
  1. 1.Laboratory of Conditioned Reflex Neurochemistry, Institute of Higher Nervous Activity and NeurophysiologyRussian Academy of SciencesMoscow
  2. 2.Laboratory of Viral Genome Cloning, Institute of Viral PreparationsRussian Academy of Medical SciencesMoscow
  3. 3.Laboratory of Neuroimmunology, Mental Health CenterRussian Academy of Medical SciencesMoscow

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