Abstract
We have determined the molecular maps of theH-2D andQa regions of thet-complex haplotypest l2 andt w5 by chromosomal walking. Analysis with class I probes and other probes unique to theH-2D:Qa subregion indicates that the class I gene organization oft 12 is:D1-D2-Q1-Q2-Q3-Qx-Q4-Q5-Q10, while that oft w5 is:D1-D2-Q1-Q2-Q4-Q5-Q10. Thus, the absence of theQ6-Q9 genes suggested previously int-haplotypes was confirmed. A comparison of the molecular maps of thet 12 andt w5 chromosomes revealed an extremely mosaic pattern of diversity: The regions betweenD1 andD2, and betweenQ4 andQ10, are very similar in both chromosomes. However, theirQ1 toQ3 regions are strikingly different. Further comparisons of wild-type chromosomes and additionalt-haplotypes by molecular mapping and genomic Southern blot hybridization with probes to theQ1-Q3 region showed a high level of polymorphism among both wild-type chromosomes and amongt-haplotypes. The characteristics of the polymorphisms suggest that recombination may play an important role in generating this genetic diversity. Furthermore, recombination between wild-type andt-haplotype chromosomes may be involved.
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Uehara, H., Abe, K., Flaherty, L. et al. Molecular organization of theD-Qa region oft-haplotypes suggests that recombination is an important mechanism for generating genetic diversity of the major histocompatibility complex. Mammalian Genome 1, 92–99 (1991). https://doi.org/10.1007/BF02443784
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DOI: https://doi.org/10.1007/BF02443784