Mammalian Genome

, 1:84 | Cite as

Genetic mapping of thet-complex region on mouse chromosome 17 including theHybrid sterility-1 gene

  • Jiři Forejt
  • Vladimir Vincek
  • Jan Klein
  • Hans Lehrach
  • Milada Loudová-Micková
Original Contributions


t-haplotypes occupy a region on chromosome (Chr) 17 which slightly overlaps the ends of theT-H-2 interval. The wild-type form of this 14 centi-Morgan (cM) region was mapped in a multilocus backcross (C57BL/10-T×C3H)F1×C57BL/10 using 15 DNA probes on Southern blots of the DNA extracted from 53 animals which were recombinants in theT-H-2 interval. Each recombinant was also progenytested to ascertain itsHybrid sterility-1 (Hst-1) genotype by crossing to PWB/Ph, aMus musculus-derived inbred strain. The limit of resolution of the cross was 0.27 cM. The map distances have been determined for the DNA loci in theT-H-2 interval and theHst-1 gene was mapped in close vicinity to theD17Rp17 locus.


Inbred Strain Recombinant Inbred Hybrid Sterility Recombinant Inbred Strain Wild Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Artzt, K.: Gene mapping within theT/t complex of the mouse. III.t-lethal genes are arranged in three clusters on chromosome 17.Cell 39: 565–572, 1984.PubMedCrossRefGoogle Scholar
  2. Bučan, M., Herrmann, B.G., Frischauf, A.M., Bautch, V.L., Bode, V., Silver, L.M., Martin, G.R. and Lehrach, H.: Deletion and duplication of DNA sequence is associated with the embryonic lethal phenotype of the t9 complementation group of the mouset-complex.Genes Develop 1: 376–395, 1987.PubMedGoogle Scholar
  3. Bullard, D.C. and Schimenti, J.C.: Molecular cloning and genetic mapping of thet-complex responder candidate gene family.Genetics 124: 957–966, 1990.PubMedGoogle Scholar
  4. Coyne, J.A.: Genetic studies of three sibling species ofDrosophila with relationship to theories of speciation.Genet Res 46: 169–192, 1985.PubMedGoogle Scholar
  5. Coyne, J.A. and Barton, N.H.: What do we know about speciation?Nature 331: 485–486, 1988.CrossRefGoogle Scholar
  6. Coyne, J.A. and Orr, H.A.: Two rules of speciation.In D. Otte and J.A. Endler (eds.); Speciation and its consequences, pp. 180–207, Sinauer Associates, Sunderland MA, 1989.Google Scholar
  7. Deimling, von O.H., Forejt, J. and Wienker, T.F.: Allelic profile at 37 biochemical loci of two inbred strains of the mice derived from wildMus musculus.Laborat Anim 22: 61–66, 1988.Google Scholar
  8. Dobzhansky, T.:Genetics and the Origin of Species, Third Edition, Columbia University Press, New York, 1951.Google Scholar
  9. Dunn, L.C.: Linkage in mice and rats.Genetics 5: 325–343, 1920.PubMedGoogle Scholar
  10. Fasano, O., Birnbaum, D., Edlund, L., Fogh, J. and Wigler, M.: New human transforming genes detected by a tumorigenicity assay.Mol Cell Biol 4: 1695, 1984.PubMedGoogle Scholar
  11. Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal Biochem 132: 6–13, 1983.PubMedCrossRefGoogle Scholar
  12. Figueroa, F., Vincek, V., Kasahara, M., Bell, G.I. and Klein, J.: Mapping of theSod-2 locus into thet-complex on mouse chromosome 17.Immunogenetics 28: 260–264, 1988.PubMedCrossRefGoogle Scholar
  13. Forejt, J.: Hybrid sterility gene located in theT/t-H-2 supergene on chromosome 17.In S. Reisfeld and R.A. Ferone (eds.),Current Trends in Histocompatibility, pp. 103–131. Plenum Press, New York, 1981.Google Scholar
  14. Forejt, J.: Chromosomal and genic sterility of hybrid type in mice and man.Exp Clin Immunogenet 2: 106–119, 1985.PubMedGoogle Scholar
  15. Forejt, J. and Iványi, P.: Genetic studies on male sterility of hybrids between laboratory and wild mice (Mus musculus L.).Genet Res 24: 189–206, 1975.CrossRefGoogle Scholar
  16. Fox, H.S., Martin, G.R., Lyon, M.F., Herrmann, B., Frischauf, A.M., Lehrach, H. and Silver, L.M.: Molecular probes define different regions of the mouset-complex.Cell 40: 63–69, 1985.PubMedCrossRefGoogle Scholar
  17. Haldane, J.B.S.: Sex ratio and unisexual sterility in hybrid animals.J Genet 12: 101–109, 1922.CrossRefGoogle Scholar
  18. Hammer, M.F., Schimenti, J. and Silver, L.M.: Evolution of mouse chromosome 17 and the origin of inversions associated witht-haplotypes.Proc Natl Acad Sci USA 86: 3261–3265, 1989.PubMedCrossRefGoogle Scholar
  19. Herrmann, B.G. and Lehrach, H.: From phenotype to gene: Molecular cloning in theBrachyury (T) locus region.Curr Top Microbiol Immunol 137: 77–81, 1988.PubMedGoogle Scholar
  20. Herrmann, B.G., Bučan, M., Main, P.E., Frischauf, A.M., Silver, L.M. and Lehrach, H.: Genetic analysis of the proximal portion of the mouset-complex: Evidence for a second inversion withint-haplotypes.Cell 44: 469–476, 1986.PubMedCrossRefGoogle Scholar
  21. Herrmann, B.G., Barlow, D. and Lehrach, H.: A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximalt-complex inversion.Cell 48: 813–825, 1987.PubMedCrossRefGoogle Scholar
  22. Herrmann, B.G., Labeit, S., Poustka, A., King, T.R. and Lehrach, H.: Cloning of theT gene required in mesoderm formation in the mouse.Nature 343: 617–622, 1990.PubMedCrossRefGoogle Scholar
  23. Iványi, P., Vojtíšková, M., D''emant, P. and Micková, M.: Genetic factors in the ninth linkage group influencing reproductive performance in male mice.Folia Biol 15: 401–421, 1969.Google Scholar
  24. King, T.R., Dove, W.R., Herrmann, B., Moser, A.R. and Shedlovsky, A.: Mapping to molecular resolution in theT toH-2 region of the mouse genome with a nested set of meiotic recombinants.Proc Natl Acad Sci USA 86: 222–226, 1989.PubMedCrossRefGoogle Scholar
  25. Klein, J.:Biology of the Mouse Histocompatibility-2 Complex: Principles of Immunogenetics Applied to a Single System, Springer-Verlag. New York, 1975.Google Scholar
  26. Klein, J.,Natural History of the Major Histocompatibility Complex, John Wiley and Sons, New York, 1986.Google Scholar
  27. Kvist, S., Roberts, L. and Dobberstein, B.: Mouse histocompatibility genes: Structure and organization of aK d gene.EMBO J 2: 245–254, 1983.PubMedGoogle Scholar
  28. Leder, A., Swan, D., Ruddle, F., D'Eustachio, P. and Leder, P.: Dispersion of α-like globin genes of the mouse to three different chromosomes.Nature 293: 196–200, 1981.PubMedCrossRefGoogle Scholar
  29. Lewontin, R.C.:Genetic Basis of Evolutionary Change, Columbia University Press, New York, 1974.Google Scholar
  30. Mayr, E.:Animal Species and Evolution, Harvard University Press, Cambridge, 1963.Google Scholar
  31. Mann, E.A., Silver, L.M. and Elliott, R.W.: Genetic analysis of a mouseT complex locus that is homologous to a kidney cDNA clone.Genetics 114: 993–1006, 1986.PubMedGoogle Scholar
  32. Micková, M. and Iványi, P.: Study ofH-2 antigens of wild mice (Mus musculus L.).Folia Biol 22: 159–189, 1976.Google Scholar
  33. Mosinger, B., Králová, J. and Forejt, J.: A clonedH-2 class I gene from at w32-derived recombinantt-haplotype identified as functionalH-2K q gene.Immunogenetics 28: 283–285, 1988.PubMedCrossRefGoogle Scholar
  34. Nadeau, J.H. and Phillips, S.J.: The putative oncogenePim-1 in the mouse: Its linkage and variation amongt-haplotypes.Genetics 117: 533–541, 1987.PubMedGoogle Scholar
  35. Oi, V., Jones, P.P., Goding, J.W., Herzenberg, L.A. and Herzenberg, L.A.: Properties of monoclonal antibodies to mouseIg antigens,H-2 andIa antigens.Curr Top Microbiol Immunol 81: 115–129, 1978.PubMedGoogle Scholar
  36. Pavljuková, H. and Forejt, J.: Private communication.Mouse News Lett 65: 44, 1981.Google Scholar
  37. Rappold, G.A., Stubbs, L., Labeit, S., Crkvenjakov, R.B. and Lehrach, H.: Identification of a testis-specific gene from mouset-complex next to aCpG-rich island.EMBO J 6: 1975–1980, 1987.PubMedGoogle Scholar
  38. Rohme, D., Fox, H., Herrmann, B., Frischauf, A.M., Edstrom, J.-E., Mains, P., Silver, L.M. and Lehrach, H.: Molecular clones of the mouset-complex derived from microdissected metaphase chromosomes.Cell 36: 783–788, 1984.PubMedCrossRefGoogle Scholar
  39. Sarvetnick, N., Fox, H.S., Mann, E., Mains, P.E., Elliott, R.W. and Silver, L.M.: Nonhomologous pairing in mice heterozygous for at-haplotype can produce recombinant chromosomes with duplications and deletions.Genetics 113: 723–734, 1986.PubMedGoogle Scholar
  40. Schimenti, J., Vold, L., Socolow, D. and Silver, L.M.: An unstable family of large DNA elements in the center of the mouset-complex.J Mol Biol 195: 583–594, 1987.CrossRefGoogle Scholar
  41. Schimenti, J., Cebra-Thomas, J., Decker, C.L., Islam, S.D., Pilder, S.H. and Silver, L.M.: A candidate gene family for the mouset-complex responder locus responsible for haploid effects on sperm function.Cell 55: 71–78, 1988.PubMedCrossRefGoogle Scholar
  42. Silver, L.M.: Mouset-haplotypes.Ann Rev Genet 19: 179–208, 1985.PubMedGoogle Scholar
  43. Skow, L.C. and Donner, M.E.: The locus encoding αA-crystallin is closely linked toH-2 k on mouse chromosome 17.Genetics 110: 723–732, 1985.PubMedGoogle Scholar
  44. Vincek, V., Kawaguchi, H., Mizuno, K., Zaleska-Rutczynska, Z., Kasahara, M., Forejt, J., Figueroa, F. and Klein, J.: Linkage map of mouse chromosome 17: Localization of 27 new DNA markers.Genomics 5: 773–786, 1989.PubMedCrossRefGoogle Scholar
  45. Willison, K.R., Dudley, K. and Potter, J.: Molecular cloning and sequence analysis of a haploid expressed gene encodingt-complexpolypeptide-1.Cell 44: 727–738, 1986.PubMedCrossRefGoogle Scholar
  46. Winking, H. and Silver, L.M.: A recombinant mouset-haplotype that expresses a dominant lethal maternal effect.Genetics 108: 1013–1020, 1984.PubMedGoogle Scholar
  47. Xiang, K., Cox, N.J., Hallewell, R.A. and Bell, G.I.: MultipleTaq I RFLPs at the humanmanaganese superoxide dismutase (Sod-2) locus on chromosome 6.Nucl Acid Res 15: 7654, 1987.Google Scholar

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Jiři Forejt
    • 1
  • Vladimir Vincek
    • 2
  • Jan Klein
    • 2
  • Hans Lehrach
    • 3
  • Milada Loudová-Micková
    • 1
  1. 1.Institute of Molecular GeneticsCzechoslovak Academy of SciencesPrague 4Czechoslovakia
  2. 2.Abteilung ImmungenetikMax-Planck-Institut für BiologieTübingenGermany
  3. 3.Imperial Cancer Research Fund LaboratoriesLondonUK

Personalised recommendations