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Letters in Peptide Science

, Volume 7, Issue 1, pp 23–26 | Cite as

Synthesis of chicken galanin and its N- and C-terminal segments, and preparation of their antisera

  • Lajos Baláspiri
  • Zsuzsanna Tegyei
  • Maria Dux
  • Gábor Jancsó
  • Rita Józsa
  • Valér Csernus
  • Béla Mess
Article
  • 27 Downloads

Summary

We describe the synthesis of the first avian galanin (GAL), chicken GAL, and its N-terminal and C-terminal segments by solid-phase synthesis, using Boc/Bzl amino acid protection groups and MBHA resin. The three peptides were prepared with purities of over 97%, as determined by RP-HPLC, HPCE, FAB-MS or ESI-MS and amino acid analysis. Antibodies against these synthetic peptides were raised in rabbits and used for immunohistochemical localization of GAL-immunoreactive neurons in chicken brain.

Key words

antibodies chicken galanin segments synthesis 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Lajos Baláspiri
    • 1
  • Zsuzsanna Tegyei
    • 1
  • Maria Dux
    • 2
  • Gábor Jancsó
    • 2
  • Rita Józsa
    • 3
  • Valér Csernus
    • 3
  • Béla Mess
    • 3
  1. 1.Central Research Institute for Chemistry of Hungarian Academy of SciencesBudapestHungary
  2. 2.Institute of PhysiologyAlbert Szent-Györgyi Medical UniversitySzegedHungary
  3. 3.Department of AnatomyMedical University of PécsPécsHungary

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