Synthesis of chicken galanin and its N- and C-terminal segments, and preparation of their antisera
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We describe the synthesis of the first avian galanin (GAL), chicken GAL, and its N-terminal and C-terminal segments by solid-phase synthesis, using Boc/Bzl amino acid protection groups and MBHA resin. The three peptides were prepared with purities of over 97%, as determined by RP-HPLC, HPCE, FAB-MS or ESI-MS and amino acid analysis. Antibodies against these synthetic peptides were raised in rabbits and used for immunohistochemical localization of GAL-immunoreactive neurons in chicken brain.
Key wordsantibodies chicken galanin segments synthesis
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- 3.Baláspiri, L., Janáky, T., Tegyei, Zs., Krajcsovits, F., Blazsó, G., Mák, M., Takács, T. and Kása, P., In Epton, R. (Ed.), Proceedings of the 5th Symposium of Solid-Phase Synthesis, London, U.K., 1999.Google Scholar
- 12.Stewart, J. and Young, J., Solid Phase Peptide Synthesis, W.H. Freeman and Company, San Francisco, CA, 1969.Google Scholar
- 13.Sternberger, L. A., Hardy Jr., P. H., Cucullis, J. J. and Meyer, H. G., Immunochemistry, Wiley, New York, NY, 1986, pp. 1–524.Google Scholar