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1H- and13C-NMR spectroscopic studies of lipid extracts of the red algaGracilaria longa

Abstract

Lipid extracts of the red algaGracilaria longa were studied by1H- and13C-NMR spectroscopy. Peaks in the13C-NMR spectra attributable to sterols, chlorophylls and carotenoids allowed free and acylated cholesterol, chlorophylla and lutein to be identified as the most abundant components of these classes. A content of 0.5 ± 0.1 μmoles of total cholesterol/g wet alga was estimated from the1H-NMR spectrum, which also allowed the determination of the phosphatidylcholine/total lipid molar ratio (9.5 ± 0.5%). The13C-NMR spectroscopic experiments provided information on the position of the double bonds on the fatty acid residues. A comparison between NMR spectra of lipid extracts obtained for wet and dried alga showed that the alga undergoes both a dramatic peroxidation and some glycolipid degradation during the drying process.

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Pollesello, P., Toffanin, R., Murano, E. et al. 1H- and13C-NMR spectroscopic studies of lipid extracts of the red algaGracilaria longa . J Appl Phycol 4, 149–155 (1992). https://doi.org/10.1007/BF02442463

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  • DOI: https://doi.org/10.1007/BF02442463

Key words

  • red alga
  • lipid extract
  • 1H-NMR
  • 13C-NMR
  • Gracilaria longa