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1H- and13C-NMR spectroscopic studies of lipid extracts of the red algaGracilaria longa

Journal of Applied Phycology volume 4, pages 149–155 (1992)Cite this article

Abstract

Lipid extracts of the red algaGracilaria longa were studied by1H- and13C-NMR spectroscopy. Peaks in the13C-NMR spectra attributable to sterols, chlorophylls and carotenoids allowed free and acylated cholesterol, chlorophylla and lutein to be identified as the most abundant components of these classes. A content of 0.5 ± 0.1 μmoles of total cholesterol/g wet alga was estimated from the1H-NMR spectrum, which also allowed the determination of the phosphatidylcholine/total lipid molar ratio (9.5 ± 0.5%). The13C-NMR spectroscopic experiments provided information on the position of the double bonds on the fatty acid residues. A comparison between NMR spectra of lipid extracts obtained for wet and dried alga showed that the alga undergoes both a dramatic peroxidation and some glycolipid degradation during the drying process.

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Authors and Affiliations

  1. POLY-biòs Research Center, LBT, Area di Ricerca, Padriciano 99, I-34012, Trieste, Italy

    Piero Pollesello, Renato Toffanin, Erminio Murano & Bjarne J. Kvam

  2. Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via A. Valerio 38, I-34127, Trieste, Italy

    Roberto Rizzo & Sergio Paoletti

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  1. Piero Pollesello
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  2. Renato Toffanin
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  3. Erminio Murano
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  4. Roberto Rizzo
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  5. Sergio Paoletti
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  6. Bjarne J. Kvam
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Pollesello, P., Toffanin, R., Murano, E. et al. 1H- and13C-NMR spectroscopic studies of lipid extracts of the red algaGracilaria longa . J Appl Phycol 4, 149–155 (1992). https://doi.org/10.1007/BF02442463

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  • DOI: https://doi.org/10.1007/BF02442463

Key words

  • red alga
  • lipid extract
  • 1H-NMR
  • 13C-NMR
  • Gracilaria longa