Isoelectric focusing of human head hair proteins. Preliminary research
The examination of human hair keratin to obtain genetic information, which may be useful also in forensic sciences, has been carried out with the use of isoelectrophoretic procedure obtaining considerable evidence for the existence of specific-species patterns.
In this paper the keratins extracted from hairs of 280 subjects belonging to Sardinian people (113 males, 167 females, aged from 1 to 89, belonging to 52 families) were analyzed using IEF in thin-layer polyacrylamide gel (0.5 mm) in the pH range 2.5–7.0, followed by the silver staining method.
Number, position and colour of the bands were the same in all the analyzed samples but a large individual variability was revealed for the relative intensity of some bands.
Differences for a long time storage were not revealed as well hair's sample as protein extract: Neither were differences in the number and position of the bands analyzing samples of hair from several sites of the head of the same individual revealed.
The results obtained are a useful indication to continue this research considering the numerous fields of application of this analysis system.
Key wordskeratins hairs isoelectric focusing
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