International Journal of Anthropology

, Volume 5, Issue 1, pp 25–31 | Cite as

Isoelectric focusing of human head hair proteins. Preliminary research

  • M. Folin
  • A. Cau
Article

Abstract

The examination of human hair keratin to obtain genetic information, which may be useful also in forensic sciences, has been carried out with the use of isoelectrophoretic procedure obtaining considerable evidence for the existence of specific-species patterns.

In this paper the keratins extracted from hairs of 280 subjects belonging to Sardinian people (113 males, 167 females, aged from 1 to 89, belonging to 52 families) were analyzed using IEF in thin-layer polyacrylamide gel (0.5 mm) in the pH range 2.5–7.0, followed by the silver staining method.

Number, position and colour of the bands were the same in all the analyzed samples but a large individual variability was revealed for the relative intensity of some bands.

Differences for a long time storage were not revealed as well hair's sample as protein extract: Neither were differences in the number and position of the bands analyzing samples of hair from several sites of the head of the same individual revealed.

The results obtained are a useful indication to continue this research considering the numerous fields of application of this analysis system.

Key words

keratins hairs isoelectric focusing 

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References

  1. Budowle B. & Acton R.T., 1981.Isoelectric focusing of hair Proteins. Proceedings of the 3rd International Conference, Charleston, S.C., USA.Google Scholar
  2. Carracedo A., Concheiro L. &Requena I., 1985.The isoelectric focusing of keratins in hair followed by silver staining. Forensic Sciences International, 29:83–89.CrossRefGoogle Scholar
  3. Carracedo A., Prieto J.M., Concheiro L. &Estefania J., 1987.Isoelectric focusing patterns of some mammalian keratins. Journal of Forensic Sciences, 32: 93–99.Google Scholar
  4. Darskus R.L. &Gillespie J.M., 1971.Cited by Carracedo et al., 1985. Forensic Sciences International, 29: 83–89.Google Scholar
  5. Gerhard M., 1987.Electrophoretic variability in human head hair: polyacrylamide gel electrophoresis of hair proteins in the presence of sodium dodecyl sulfate and urea. Electrophoresis, 8: 153–157.CrossRefGoogle Scholar
  6. Hrdy D.B., Baden H.P., Lee D.L., Kubilus J., Ludwig W.K., 1977.Frequency of an electrophoretic variant of hair keratin human population. American J. of Human Gen., 29: 98–100.Google Scholar
  7. Marshall R.C., Frenkel M.J. &Gillespiej, M., 1977.High sulfur proteins in mammalian keratins: a possible aid in classification. Austr. Jour. of Zoology, 25: 121–132.CrossRefGoogle Scholar
  8. Marshall R.C., Gillespie J.M. &Klement V., 1985.Methods and future prospects for forensic identification of hair by electrophoresis. Journal of Forensic Sci. Soc., 25: 57–66.CrossRefGoogle Scholar
  9. Marshall T. &Williams K.M., 1986.High resolution two dimensional electrophoresis of hair proteins without prior S-carboxymethylation. Electrophoresis, 7: 524–526.CrossRefGoogle Scholar

Copyright information

© Editrice Il Sedicesimo 1990

Authors and Affiliations

  • M. Folin
    • 1
  • A. Cau
    • 1
  1. 1.Department of BiologyUniversity of PaduaPaduaItaly

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