Abstract
In step with the increased use of platelet concentrates, the lack of objective and reproducible methods to assess platelet viability in unopened packs has become more apparent. The paper describes a computerised method for monitoring changes in light transmission through platelet packs under continuous rotation and for the entire storage period. The method was tested on 24 platelet concentrates, which were monitored for varying periods of time. In all concentrates tested a distinct time could be defined where a phase with gradually increased light transmission through the concentrates started. Furthermore, 35 platelet concentrates stored in Pl-146 plastic packs were examined and divided into two groups and observed for three and four days, respectively. These concentrates were monitored continuously and subsequently compared with parameters believed to reflect the quality of platelet concentrates. Concentrates in both groups showing an early increase in light transmission were found to have lower pH values at the end of the observation periods.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bellhouse, M. A., Ross, I., Entwistle, C. C. andBellhouse, B. J. (1985) Optical measurement of the viability of stored human platelets.Opt. & Laser Technol.,17, 27–30.
Eriksson, L., Högman, C. F. andBusch, C. (1981) Lack of conformity in the behaviour of platelets during normal storage conditions at 22°C.Vox Sang.,40, 65–70.
Fratantoni, J. C., Poindexter, B. J. andBonner, R. F. (1984) Quantitative assessment of platelet morphology by light scattering. A potential method for the evaluation of platelets for transfusion.J. Lab. Clin. Med.,103, 620–631.
Frojmovic, M. M. andPanjwani, R. (1975) Blood cell structure—function studies: light transmission and attenuation coefficients of suspensions of blood cells and model particles at rest and with stirring.,86, 326–347.
Gullikson, H. (1985) Preservation of red blood cells and platelets. Thesis, ISBN 91-7222-918-7, Part VI. Karolinska Institute, Stockholm.
Hampshire, N. (1982)Vic revealed. Duckworth, London.
Holme, S. andMurphy, S. (1972) Quantitative measurement of platelet shape by light transmission studies; application to storage of platelets for transfusion.J. Lab. Clin. Med.,92, 53–64.
Holme, S. andMurphy, S. (1978) Coulter counter and light transmission studies of platelets exposed to low temperature, ADP, EDTA and storage at 22°.,96, 480–493.
Holme, S., Simmonds, M., Ballek, R. andMurphy, S. (1981) Comparative measurements of platelet size by Coulter Counter, microscopy of blood smears, and light transmissions studies.,97, 610–622.
Latimer, P., Born, G. V. R. andMichal, F. (1978) Application of the light scattering theory to the optical effects associated with the morphology of blood platelets.Arch. Biochem. Biophys.,180, 151–159.
Latimer, P. (1979) Light scattering vs microscopy for measuring average cell size and shape.Biophys. J.,27, 117–126.
Solberg, C., Holme, S. andLittle, C. (1986) Morphological changes associated with pH changes during storage of platelets concentrates in first-generation 3-day container.Vox Sang.,50, 71–77.
Trenchard, P. M. (1986) Continuous monitoring of platelet morphology during small-scale in vitro storage.,51, 185–191.
van de Hulst, H. C. (1957)Light scattering by small particles. John Wiley & Sons, New York.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Järemo, P. Computerised method for monitoring stored platelet packs. Med. Biol. Eng. Comput. 26, 193–198 (1988). https://doi.org/10.1007/BF02442263
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02442263