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Calcified Tissue International

, Volume 28, Issue 1, pp 113–119 | Cite as

The influence of calcitonin on the plasma and urine phosphate changes produced by parathyroid hormone

  • Roy V. Talmage
  • Carole J. VanderWiel
Laboratory Investigations

Summary

The influence of calcitonin (CT) on parathyroid hormone-induced changes in plasma phosphate, urine phosphate, plasma32P and32P specific activity (32P-S.A.) was studied using thyroparathyroidectomized rats. Radiophosphorus was injected at either 10 h (32P-10 h) or 8 days (32P-8 d) before commencing hormonal treatment. In order to produce consistent plasma32P-S.A. changes in rats treated with parathyroid hormone (PTH) alone, we maintained all animals for 48 h prior to hormone injection on a closely regulated carbohydrate intake (both quantity of food and time of feeding). Rats were then fasted 10 h prior to injection of PTH (0.01 U/g body weight/h), or CT (0.1 mU/g body weight/h), or both hormones injected simultaneously. Under these conditions, PTH produced a rise (relative to controls) in plasma32P-S.A. (-8 d) during an 8-h experimental period, while plasma32P-S.A. (-10 h) fell. Calcitonin treatment alone did not affect plasma32P-S.A., regardless of when32P was administered.

When both hormones were injected concurrently, CT augmented the PTH-induced hypophosphatemia and magnified the fall in plasma32P levels, but the addition of CT did not affect PTH-induced changes in either renal calcium or phosphate excretion. However, the PTH-induced changes in plasma32P-S.A. were abolished.

These data could not be explained by the opposing effects of the two hormones on rates of bone resorption. We interpret the results as adding support to the postulate that calcitonin moves phosphate into and prevents its loss from bone and bone fluid.

Key words

Parathyroid hormone Calcitonin Plasma phosphate Bone phosphate Plasma32P specific activity 

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References

  1. 1.
    Copp, D.H., Cameron, E.C., Cheney, B.A., Davidson, A.G.F., Henze, K.G.: Evidence for calcitonin: a new hormone from the parathyroid that lowers blood calcium, Endocrinology70:638–649, 1962PubMedGoogle Scholar
  2. 2.
    Hirsch, P.F., Gauthier, G.F., Munson, P.L.: Thyroid hypocalcemic principle and recurrent laryngeal nerve injury as factors affecting the response to parathyroidectomy in rats, Endocrinology73:244–252, 1963PubMedGoogle Scholar
  3. 3.
    Hirsch P.F., Voelkel, E.F., Munson, P.L.: Thyrocalcitonin: hypocalcemic, hypophosphatemic principle of the thyroid gland, Science146:412–413, 1964PubMedGoogle Scholar
  4. 4.
    Kenny, A.D.: Discussion of paper by D.H. Copp, Recent Prog. Horm. Res.20:84, 1964Google Scholar
  5. 5.
    Talmage, R.V., Anderson, J.J.B.: The effect of calcitonin on32P disappearance from plasma in parathyroidectomized and nephrectomized rats, Proc. Soc. Exp. Biol. Med.141:982–985, 1972PubMedGoogle Scholar
  6. 6.
    Talmage, R.V., Anderson, J.J.B., Cooper, C.W.: The influence of calcitonin on the disappearance of radiocalcium and radiophosphorus from plasma, Endocrinology90:1185–1193, 1972PubMedGoogle Scholar
  7. 7.
    Talmage, R.V., Whitehurst, L.A., Anderson, J.J.B.: Effect of calcitonin and calcium infusion on plasma phosphate, Endocrinology92:792–798, 1973PubMedCrossRefGoogle Scholar
  8. 8.
    Johnston, C.C., Deiss, W.P.: An inhibitory effect of thyrocalcitonin on calcium releasein vivo on bone metabolismin vitro, Endocrinology78:1139–1143, 1966PubMedCrossRefGoogle Scholar
  9. 9.
    O'Riordan, J.L.H., Aurbach, G.D.: Mode of action of thyrocalcitonin, Endocrinology82:377–383, 1968PubMedGoogle Scholar
  10. 10.
    Talmage, R.V., Doppelt, S.H., and Postma, J.H.: Observations on the relationship of parathyroid hormone and calcitonin to plasma and liver phosphate, Proc. Soc. Exp. Biol. Med.153:131–137, 1976PubMedGoogle Scholar
  11. 11.
    Carnes, D.L., Campbell, J.W.: Incorporation of purines and purine precursors into the nucleotide pool of isolated bone cells, Int. J. Biochem.9:517–529, 1978PubMedCrossRefGoogle Scholar
  12. 12.
    Talmage, R.V., VanderWiel, C.J., Raneri, D.B.: Factors affecting parathyroid hormone-induced hypophosphatemia and32P specific activity in thyroparathyroidectomized rats, Calcif. Tissue Int.27:239–246, 1979PubMedGoogle Scholar
  13. 13.
    Meyer, R.A., Meyer, M.H.: Soft tissue phosphate loss accompanying the hypophosphaturic effect of parathyroid hormone in rats, Endocrinology94:1331–1335, 1974PubMedGoogle Scholar
  14. 14.
    Borle, A.B., Briggs, E.N.: Microdetermination of calcium in biological material by automatic fluorometric titration, Anal. Chem.40:339–344, 1968PubMedCrossRefGoogle Scholar
  15. 15.
    Chen, P.S., Toribara, R.Y., Warren, H.: Microdetermination of phosphorus, Anal. Chem.28:1756–1758, 1965CrossRefGoogle Scholar
  16. 16.
    Grubb, S.A., Markham, T.C., Talmage, R.V.: Effect of salmon calcitonin infusion on plasma concentrations of recently administered45Ca, Calcif. Tissue Res.24:201–208, 1977PubMedCrossRefGoogle Scholar
  17. 17.
    Hirsch, P.F.: Thyrocalcitonin inhibition of bone resorption induced by parathyroid extract in thyroparathyroidectomized rats, Endocrinology80:539–541, 1967PubMedGoogle Scholar
  18. 18.
    Matthews, J.L., Martin, J.H., Collins, E.J., Kennedy, J.W., Powell, E.L.: Immediate changes in the ultrastructure of bone cells following thyrocalcitonin administration. In R.V. Talmage, P.L. Munson (eds.): Calcium Parathyroid Hormone and the Calcitonins, pp. 375–382. Excerpta Medica, Amsterdam, 1972Google Scholar
  19. 19.
    Norimatsu, H. VanderWiel, C.J., Talmage, R.V.: Electron microscopic study of the effects of calcitonin on bone cells and their extracellular milieu, Clin. Orthop.139, 1979Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Roy V. Talmage
    • 1
  • Carole J. VanderWiel
    • 1
  1. 1.Department of Surgery, Orthopaedic Research LaboratoriesUniversity of North Carolina, School of MedicineChapel HillUSA

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