Uptake of47Ca into the forearm in normal subjects and in patients with osteomalacia, primary biliary cirrhosis, and hyperparathyroidism

  • R. C. Smart
  • C. D. Holdsworth
Clinical Investigations


The rate of accumulation of47Ca in the tissues of the forearm in the 24 h following an intravenous bolus injection of 2 µCi of47CaCl2 was monitored using a large-volume scintillation counter and analyzed using principal component analysis. In normal subjects activity rose rapidly to a maximum value of about 2% of the administered dose between 2 and 4 h after injection, and had declined little if at all after 24 h. The first three principal components of the uptake curves accounted for a cumulative total of 99.4% of the variance of the data. The eight patients with osteomalacia had a significantly positive weighting factor for the first component, which reflected relatively rapid uptake 15 min after injection and increased total uptake at 6 h. The 6 patients with primary biliary cirrhosis but no overt bone disease had a less marked abnormality which probably represented early osteomalacia. The 3 patients with hyperparathyroidism had normal rates of uptake. Principal component analysis made it possible to quantitate differences in the rate of calcium uptake by the forearm tissues under varying physiological conditions without the need for assumptions on compartmental distribution.

Key words

47Ca isotope Calcium kinetics Osteomalacia Primary biliary cirrhosis Hyperparathyroidism 


  1. 1.
    Corey, K.R., Weber, D., Merlino, M., Greenberg, E., Kenney, P., Laughlin, J.S.: Calcium turnover in man—dynamic clinical studies with radioisotope, AEC Symp.3:519–536, 1964Google Scholar
  2. 2.
    Wendeberg, B.: Mineral metabolism of fractures of the tibia in man studied with external counting of Sr-85, Acta Orthop. Scand. [Suppl. 52], 1961Google Scholar
  3. 3.
    Harmut, M., Paponic, S., Jobanovic, V., Simonovic, I.: Determination of calcium accretion by external measurement of47Ca, Nucl. Med. (Stuttg.)8:187–195, 1969Google Scholar
  4. 4.
    Harmut, M.: Determination of accretion rate in the body by external measurements of47Ca in the human forearm using the generalised Bauer-Carlsson-Lindquist (BCL) equation, Nucl. Med. (Stuttg.)13:186–192, 1974Google Scholar
  5. 5.
    Wendeberg, B.: Bone salt accretion and exchangeable calcium spaces in man measured with47Ca and85Sr, Clin. Orthop.40:162–183, 1965PubMedGoogle Scholar
  6. 6.
    Neer, R., Berman, M., Fisher, L., Rosenberg, L.E.: Multicompartmental analysis of calcium kinetics in normal adult males, J. Clin. Invest.46:1364–1379, 1967PubMedGoogle Scholar
  7. 7.
    Morrison, D.F.: Multivariate Statistical Methods. p. 221. McGraw-Hill, New York, 1967Google Scholar
  8. 8.
    Seal, H.L.: Multivariate Statistical Analysis for Biologists, p. 101. Methuen & Co., Ltd., London, 1964Google Scholar
  9. 9.
    Barber, D.C., Duthie, H.L., Howlett, P.J., Ward, A.S.: Principal components: a new approach to the analysis of gastric emptying. In:Dynamic Studies with Radioisotope in Medicine, Vol. I, pp. 185–196. International Atomic Energy Agency, Vienna, 1974Google Scholar
  10. 10.
    Bradley, J.V.: Distribution-Free Statistical Tests, pp. 000-000. Prentice-Hall Inc., Englewood Cliffs, N.J., 1968Google Scholar
  11. 11.
    Curtis, F.K., Fellows, H., Rich, C.: Estimation of human calcium absorption by external radioisotope counting, J. Lab. Clin. Med.69:1036–1041, 1967PubMedGoogle Scholar
  12. 12.
    Wills, M.R., Zisman, E., Wortsman, J., Evens, R.G., Pak, C.Y.C., Bartter, F.C.: The measurement of intestinal calcium absorption by external radioisotope counting: application to study of nephrolithiasis, Clin. Sci.39:95–106, 1970PubMedGoogle Scholar
  13. 13.
    Birge, S.J., Peck, W.A., Berman, M., Whedon, G.D.: Study of calcium absorption in man: a kinetic analysis and physiologic model. J. Clin. Invest.48:1705–1713, 1969PubMedCrossRefGoogle Scholar
  14. 14.
    Smart, R.C., Holdsworth, C.D.: The measurement of calcium absorption and absorption rate with an external arm radioactivity counter, Clin. Sci. Mol. Med.54:93–97, 1978PubMedGoogle Scholar
  15. 15.
    Heaney, R.P.: Evaluation and interpretation of calcium kinetic data in man, Clin. Orthop.31:153–183, 1963PubMedGoogle Scholar
  16. 16.
    Reeve, J., Veall, N.: Radioisotope studies of calcium metabolism and their interpretation, Clin. Endocrinol. Metab.1:185–196, 1972CrossRefGoogle Scholar
  17. 17.
    Anderson, J., Tomlinson, R.W.S., Osborn, S.B., Wise, M.E.: Radiocalcium turnover in man, Lanceti:930–934, 1967CrossRefGoogle Scholar
  18. 18.
    Smart, R.C.: Studies of calcium metabolism and splenic function using radioisotope clearance techniques. Ph.D. Thesis, University of Sheffield, EnglandGoogle Scholar
  19. 19.
    Kehayoglou, A.K., Holdsworth, C.D., Agnew, J.E., Whelton, N.J., Sherlock, S.: Bone disease and calcium absorption in primary biliary cirrhosis, Lanceti:715–719, 1968CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. C. Smart
    • 1
  • C. D. Holdsworth
    • 1
  1. 1.Departments of Medical Physics and MedicineHallamshire HospitalSheffieldEngland

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