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International Journal of Legal Medicine

, Volume 110, Issue 1, pp 1–4 | Cite as

Antioxidant-related enzymes in myocardial zones and human pericardial fluid in relation to the cause of death

  • V. Ramos
  • A. Valenzuela
  • E. Villanueva
  • M. T. Miranda
Original Article

Abstract

The aim of this work was to shed light on hypoxic and ischemic processes in the heart that may lead to irreversible or lethal myocardial injury. We determined malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in human cardiac tissues from 45 medico-legal autopsies of persons who died from different causes. Samples were taken from three different areas of myocardium: the anterior and posterior walls of the left ventricle, and the interventricular septum. We used light microscopy to examine the heart sections (hematoxylin-eosin and Masson's trichromic stains), and studied the K+/Na+ ratio and pericardial fluid. A decrease in GSH-Px activity was found in cases with severe atherosclerosis of the coronary artery in comparison with the group with slight or moderate atherosclerosis. Postmortem activities of GSH-Px and SOD were significantly different in the three myocaridal zones studied. An increase in GSH-Px activity in the interventricular septum was noted in cases of cardiac deaths. Antioxidant-related enzymes such as GSH-Px and SOD can therefore be regarded as new biochemical markers indicative of myocardial hypoxia. The possible applications to the postmortem diagnosis of the cause of death are discussed.

Key words

Postmortem changes Malondialdehyde Antioxidant-related exzymes Cause of death 

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References

  1. 1.
    Ambrosio G, Chiariello M (1991) Myocardial reperfusion injury: mechanisms and management. A review. Am J Med 91: 86–88CrossRefGoogle Scholar
  2. 2.
    Andersen HR, Nielsen D, Falke E (1989) Right ventricular infarction: larger enzyme release with posterior than with anterior involvement. Int J Cardiol 22: 347–355PubMedCrossRefGoogle Scholar
  3. 3.
    Arduini A, Mezzatti A, Porreca E, Lapenna D, DeJulia J, Marzio L, Polidoro G, Cuccurullo F (1988) Effects of ischemia and reperfusion on antioxidant enzymes and mitochondrial inner membrane porteins in perfused rat heart. Biochem Biophys Acta 970: 113–121PubMedCrossRefGoogle Scholar
  4. 4.
    Bird RP, Draper HH (1984) Comparative studies on different methods of malonaldehyde determination. In: Methods in Enzymology Vol 105. Academic Press, New York, pp 299–305Google Scholar
  5. 5.
    Brinkmann B, Sepulchre MA, Fechner G (1993) The application of selected histochemical and immunohistochemical markers and procedures to the diagnosis of early myocardial damage. Int J Legal Med 106: 135–141PubMedCrossRefGoogle Scholar
  6. 6.
    Buczynski A, Wachowicz B, Kediziora-Kornatowska K, Tkaczewski W (1993) Changes in antioxidant enzyme activities, aggregability and malonyldialdehyde concentration in blood platelets from patients with coronary heart disease. Atherosclerosis 100: 223–228PubMedCrossRefGoogle Scholar
  7. 7.
    Burns J, Milroy CM, Hulewicz B, West CR, Walkley SM, Roberts NB (1992) Necropsy study of association between sudden death and cardiac enzymes. J Clin Pathol 45: 217–220PubMedGoogle Scholar
  8. 8.
    Camps FE, Robinson AE, Lucas BGB (1976) Gradwohl's Legal Medicine, 3rd edn. John Wright, Bristol, pp 220–254Google Scholar
  9. 9.
    Carmagnol F, Sinet PM, Jerome H (1983) Selenium-dependent and non-selenium dependent glutathione peroxidases in human tissue extracts. Biochem Biophys Acta 759: 49–57PubMedGoogle Scholar
  10. 10.
    Chaudière J (1994) Some clinical and biochemical constraints of oxidative stress in living cells. In: Rice-Evans CA, Burdon RH (eds) Free Radical Damage and its Control, Elsevier Science, New York, pp 23–64Google Scholar
  11. 11.
    Crapo JD, McCord JM, Fridovich I (1978) Preparation and assay of superoxide dismutase. In: Methods in Enzymology, Vol 53, Academic Press, Orlando, pp 382–393Google Scholar
  12. 12.
    Ferrari R, Ceconi C, Curello S, Cargnoni A, Alfieri O, Pardini A, Maezollo P, Visioli O (1991) Oxygen free radicals and myocardial damage: protective role of thiol-containing agents. Am J Med 91: 95–105CrossRefGoogle Scholar
  13. 13.
    Flaherty JT (1991) Myocardial injury mediated by oxygen free radicals. Am J Med 91: 79–85CrossRefGoogle Scholar
  14. 14.
    Hackel DB, Reimer KA (1990) Consequences of coronary artery disease. In: Kissane JM (ed) Anderson's Pathology, 9th edn. Mosby, St. Louis, pp 624–715Google Scholar
  15. 15.
    Halliwell B (1991) Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 91: 14–22CrossRefGoogle Scholar
  16. 16.
    Hougen HP, Valenzuela A, Lachica E, Villanueva E (1992) Sudden cardiac death: a comparative study of morphological, histochemical and biochemical methods. Forensic Sci Int 52: 161–169PubMedCrossRefGoogle Scholar
  17. 17.
    Kaijser L, Ericsson M, Walldius G (1989) Fatty acid turnover in the ischaemic compared to the non-ischaemic human heart. Mol Cell Biochem 88: 181–184PubMedCrossRefGoogle Scholar
  18. 18.
    Kogure K, Watson BD, Busto R, Abe K (1982) Potentiation of lipid peroxides by ischemia in rat brain. Neurochem Res 7: 437–454PubMedCrossRefGoogle Scholar
  19. 19.
    Lachica E, Villanueva E, Luna A (1988) Comparison of different techniques for the postmortem diagnosis of myocardial infarction. Forensic Sci Int 38: 21–26PubMedCrossRefGoogle Scholar
  20. 20.
    Lachica E, Villanueva E, Luna A (1988) Regional study of free fatty acids and free carnitine behavior in cardiac tissue in relation to different causes of death. J Forensic Sci 33: 1483–1490PubMedGoogle Scholar
  21. 21.
    Lachica E, Villanueva E, Luna A (1988) Regional distribution of total lipids, free fatty acids and free carnitine in human heart. Rev Esp Fisiol 44: 401–406PubMedGoogle Scholar
  22. 22.
    Lie JT, Titus JL (1975) Pathology of the myocardium and the conduction system in sudden coronary death. Circulation 52: 41–52Google Scholar
  23. 23.
    Luna A, Carmona A, Villanueva E (1983) The postmortem determination of CK isoenzymes in the pericardial fluid in various causes of death. Forensic Sci Int 22: 23–30PubMedCrossRefGoogle Scholar
  24. 24.
    Marklund S (1980) Distribution of CuZn superoxide dismutase and Mn superoxide dismutase in human tissues and extracellular fluids. Acta Physiol Scand Suppl 492: 19–23PubMedGoogle Scholar
  25. 25.
    Marttila RJ, Röyttä M, Lorentz H, Rinne UK (1988) Oxygen toxicity protecting enzymes in the human brain. J Neural Transm 74: 87–95PubMedCrossRefGoogle Scholar
  26. 26.
    McCord JM (1985) Oxygen-derived free radicals in post-ischaemic tissue injury. New Engl J Med 76: 159–163Google Scholar
  27. 27.
    Olson E (1962) Physiology of cardiac muscle. In: Handbook of Physiology, Vol 1. American Physiological Society, Washington DC, pp 199–235Google Scholar
  28. 28.
    Opie LH (1989) Reperfusion injury and its pharmacological modification. Circulation 80: 1049–1062PubMedGoogle Scholar
  29. 29.
    Paglia DE, Valentine WN (1966) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Anal Biochem 16: 359–364CrossRefGoogle Scholar
  30. 30.
    Placer ZA, Cushman LL, Johnson BC (1966) Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem 16: 359–364PubMedCrossRefGoogle Scholar
  31. 31.
    Rammer L, Jansson O (1976) Determination of electrolytes in the myocardium as a tool for the post-mortem diagnosis of recent infarction. Forensic Sci Int 8: 127–130CrossRefGoogle Scholar
  32. 32.
    Ramos V, Valenzuela A, Villanueva E (1996) Postmortem determination of lipid peroxidation and antioxidant-related enzymes in human heart, brain, and extracellular fluids in relation to time after death. Free Radical Bio Med (Submitted for publication)Google Scholar
  33. 33.
    Reimer KA, Richard VJ, Murry CE, Ideker RE (1991) Myocardial ischemia and infarction: anatomic and biochemical substrates for ischemic cell death and ventricular arrhythmias. In: Virmani R, Atkinson JB, Fenoglio JJ (eds) Cardiovascular Pathology. Saunders, Philadelphia, pp 61–85Google Scholar
  34. 34.
    Virmani R, Ursell PC, Fenoglio JJ (1991) Examination of the heart. In: Virmani R, Atkinson JB, Fenoglio JJ (eds) Cardiovascular pathology. Saunders, Philadelphia, pp 1–20Google Scholar
  35. 35.
    Yoshida S, Busto R, Watson BD, Santiso M, Ginsberg MD (1985) Postischemic cerebral lipid peroxidation in vitro: modification by dietary vitamin E. J Neurochem 44: 1593–1601PubMedGoogle Scholar
  36. 36.
    Yu BH (1994) Cellular defenses against damage from reactive oxygen species. Physiol Rev 74: 139–162PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • V. Ramos
    • 1
  • A. Valenzuela
    • 1
  • E. Villanueva
    • 1
  • M. T. Miranda
    • 1
  1. 1.Department of Forensic MedicineUniversity of GranadaGranadaSpain

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