Skip to main content
SpringerLink
Log in

Reaggregation of etioplast lipids and the formation of prolamellar bodies and thylakoids: An ultrastructural study

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Etioplasts of dark-grownAvena sativa plants were used to prepare either saponin-free or saponin-containing prolamellar bodies. Lipid extracts from both fractions were studied in reaggregation experiments: extracts containing saponins showed liposomes as well as tubules, while saponin-free samples formed only liposomes. Purified PLB lipids in reaggregation experiments were either studied in the presence or in the absence of saponins. Best tubule formation was found with samples containing MGDG+saponin. However, the reconstruction of PLB-like structures was not possible. The long tubules, protruding from isolated PLBs, are seen as a result of the reaction of saponins (originally located in vacuoles) with MGDG.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DGDG:

digalactosyldiglyceride

EM:

electron microscope

MGDG:

monogalactosyl-diglyceride

PC:

phosphatidylcholine

PChlide:

protochlorophyllide

PCR:

protochlorophyllide reductase

PG:

phosphatidylglycerol

PLB:

prolamellar body

PT:

prothylakoid

SAE:

saponin containing acetone extract

References

  • Bergweiler P, Röper U, Lütz C (1984), Physiol. Plantarum 60: 395–400

    Article  CAS  Google Scholar 

  • De Grip WJ, Drenthe E, Van Echteld C, De Kruijff B, Verkleij A (1979), BBA 558: 330–337

    Article  PubMed  Google Scholar 

  • Kahn A, (1968a), Plant Physiol. 13: 1769–1780

    Google Scholar 

  • Kahn A, (1968b), Plant Physiol. 13: 1781–1785

    Google Scholar 

  • Kesselmeier J, Budzikiewicz H (1979), Z. Pflanzenphysiol. 91: 333–344

    CAS  Google Scholar 

  • Kesselmeier J, Laudenbach U, Urban B (1983), In: Proceed. 6th. Intern. Congress Photosynthesis, Sybesma C (ed.), W. Junk Publ. The Hague, in press

  • Kirk JTO, Tilney-Bassett R (1978), The Plastids. Elsevier/North Holland, Amsterdam

    Google Scholar 

  • Lütz C, Kesslmeier J, Ruppel HG (1977), Z. Pflanzenphysiol. 85: 327–340

    Google Scholar 

  • Lütz C (1981a), Protopl. 108: 99–115

    Article  Google Scholar 

  • Lütz C (1981b), Z. Pflanzenphysiol. 104: 43–52

    Google Scholar 

  • Lütz C, Röper U, Beer NS, Griffiths T (1981), Eur. J. Biochem. 118: 347–353

    Article  PubMed  Google Scholar 

  • Lütz C, Nordmann U (1983), Z. Pflanzenphysiol. 110: 199–208

    Google Scholar 

  • Lütz C, Nordmann U, Tönissen H, Bergweiler P, Röper U (1983), In: Proceed. 6th. Intern. Congress Photosynthesis, Sybesma C (ed.), W. Junk Publishers, The Hague, in press

    Google Scholar 

  • Lütz C, Tönissen H (1984), Israel J. Botany, in press

  • Müller M (1981), Z. Naturforsch. 36c: 869–874

    Google Scholar 

  • Müller M, Nisius A (1982), Z. Naturforsch. 37c: 476–480

    Google Scholar 

  • Murakami S, Ikeuchi M (1982), In: Cell Function and Differentiation, B, pp. 13–23, A. Liss Inc. New York

    Google Scholar 

  • Nisius A, Müller M, Ruppel HG (1982), Z. Naturforsch. 37c: 251–255

    CAS  Google Scholar 

  • Quinn PJ, Gounaris K, Sen A, Williams WP (1982), In: Wintermans J, Kuiper P (eds.), Biochemistry and metabolism of plant lipids, pp 327–330, Elsevier Biomedical, Amsterdam

    Google Scholar 

  • Röper U, Bergweiler P, Lütz C (1983), Z. Pflanzenphysiol. 112: 89–93

    Google Scholar 

  • Ryberg M, Sundqvist C (1982), Physiol. Plant. 56: 125–132

    Article  CAS  Google Scholar 

  • Ryberg M, Sandelius AS, Selstam E (1983), Physiol. Plant. 57: 555–560

    Article  CAS  Google Scholar 

  • Selstam E, Lindblom G, Brentel I, Ryberg M (1982), In: Wintermans J, Kuiper P (eds.), Biochemistry and metabolism of plant lipids, pp 389–392, Elsevier Biomedical, Amsterdam

    Google Scholar 

  • Sen A, Williams WP, Brain AP, Quinn PJ (1982), BBA 685: 297–306

    CAS  Google Scholar 

  • Shipley GG, Green JP, Nichols BW (1973), BBA 311: 531–544

    PubMed  CAS  Google Scholar 

  • Sprague SG, Staehelin LA (1983), In: Biosynthesis and Function of plant lipids. Thomson WW, Mudd JB, Gibbs M (eds.), American Soc. Plant Physiol., Rockville, pp. 144–159

    Google Scholar 

  • Taraschi TF, De Kruiff B, Verkleij AJ, Van Echteld C (1982), BBA 685: 153–161

    Article  PubMed  CAS  Google Scholar 

  • Wellburn AR, Wellburn FAM (1972), In: Akoyunoglou G (ed.), 2nd Intern. Congress Photosynthesis, Stresa, W. Junk Publishers, The Hague, pp. 2349–2365

    Google Scholar 

  • Kesselmeier J. (1982) Protopl. 112: 127–137

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, 5000, Köln 41, FRG

    Hildegard Tönissen & Cornelius Lütz

Authors
  1. Hildegard Tönissen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cornelius Lütz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H.Keinig

In memorian of Professor Shimon Klein

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tönissen, H., Lütz, C. Reaggregation of etioplast lipids and the formation of prolamellar bodies and thylakoids: An ultrastructural study. Plant Cell Reports 3, 116–120 (1984). https://doi.org/10.1007/BF02441014

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02441014

Keywords

Search

Navigation