Plant Cell Reports

, Volume 3, Issue 3, pp 102–105 | Cite as

Somatic cell genetic studies inBrassica species

I. High frequency production of haploid plants inBrassica alba (L.) H. f. & T.
  • S. Leelavathi
  • V. S. Reddy
  • S. K. Sen
Article

Abstract

In vitro culture ofBrassica alba anthers on a growth medium containing inorganics of KB5 and organics, iron, sucrose and hormones of B5 resulted in a very high response of anthers (93.75%) towards callus induction. All the calli transferred to regeneration media responded favourably even after six months of callus induction. Numerous torpedo-shaped embryoids developed in clusters at many sites from each callus mass. Secondary embryogenesis and multiple shoot formation was also observed in many cases. The number of embryoids and plantlets produced by one embryogenic anther were as high as 169.8 and 17 respectively. 87% of the regenerated plants were haploids.

Key Words

Anther culture Brassicaalba primary and secondary embryoids 

Abbreviations

SH

Schenk and Hildebrandt, 1972

B5

Gamborg et al. 1968

NN

Nitsch and Nitsch, 1969

N6

Chu et al. 1975

MS

Murashige and Skoog, 1962

BL

Blaydes, 1966

DBM II

Gresshoff and Doy, 1972

KB5

Keller et al. 1975

BAP

6-benzyladenine

2, 4-D

2, 4-dichlorophenoxyacetic acid

p-CPA-p

chlorophenoxyacetic acid

Kin

Kinetin

NAA

naphthaleneacetic acid

IAA

indoleacetic acid

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References

  1. Anonymous (1975) Acta Bot. Sinica 17: 2Google Scholar
  2. Blaydes DF (1966) Physiol. Plant. 19: 748–753CrossRefGoogle Scholar
  3. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY (1975) Sci. Sin. 16: 659–688Google Scholar
  4. Chung CH, Jen YY, Tai WK (1977) In: Hu H (ed) Proc. of the Symp. on anther culture, Science Press, Peking, pp. 200–201Google Scholar
  5. Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158PubMedCrossRefGoogle Scholar
  6. George L, Rao PS (1982) Plant Science Letters 26: 111–116CrossRefGoogle Scholar
  7. Gresshoff PM, Doy CH (1972) Planta 107: 161–170CrossRefGoogle Scholar
  8. Hansson B (1978) Sver. Utsadesforen. Tidske. 88: 141–148Google Scholar
  9. Hoffmann F, Thomas E, Wenzel G (1982) Theor. App. Genet. 61: 225–232Google Scholar
  10. Jain SM, Suman Bagga, Neera Bhalla-Sarin, Sipra Guha-Mukherjee, Sopory SK (1980) In: Rao PS, Heble MR, Chadha MS (eds) Plant Tissue Culture, Genetic Manipulation and Somatic Hybridisation of Plant Cells, Department of Atomic Energy, Bombay, pp. 26–32.Google Scholar
  11. Kameya T, Hinata K (1970) Jpn. J. Breed. 20: 82–87Google Scholar
  12. Keller WA, Armstrong KC (1977) Can. J. Bot. 55: 1383–1388CrossRefGoogle Scholar
  13. Keller WA, Armstrong KC (1981) Can. J. of Genet. Cytol. 23: 259–265Google Scholar
  14. Keller WA, Armstrong KC, de la Roche AI (1983) In: Sen SK, Giles KL (eds) Plant Cell Culture in Crop Improvement, Plenum Press, New York, pp. 169–183Google Scholar
  15. Keller WA, Rajhathy T, Lacapra J (1975) Can. J. Genet. Cytol. 17: 655–666Google Scholar
  16. Keller WA, Stringam, GR (1978) In: Thorpe TA (ed) Frontiers of Plant Tissue Culture, Calgary, The International Association for Plant Tissue Culture, Calgary, pp. 113–122Google Scholar
  17. Klimaszewska K, Keller WA (1983) Z. Pflanzenphysiol. 109: 235–241Google Scholar
  18. Lichter R (1981) Z. Pflanzenphysiol. 103: 229–237Google Scholar
  19. Lichter R (1982) Z. Pflanzenphysiol. 105: 424–434Google Scholar
  20. Loh CS, Ingram DS (1982) New Phytologist. 91: 507–516CrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  22. Nitsch JP, Nitsch C (1969) Science 163: 85–87PubMedGoogle Scholar
  23. Quazi HM (1979) Ann. Bot. 42: 473–475Google Scholar
  24. Renard M, Dosba F (1980) Ann. Amelior. Plantes. 30: 191–209Google Scholar
  25. Satyanarayana A, Sen SK (1973) Can. J. Genet. Cytol. 15: 369–371Google Scholar
  26. Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50: 199–204Google Scholar
  27. Thomas E, Hoffmann F, Potrykus I, Wenzel G (1976) Mol. Gen. Genet. 145: 245–247CrossRefGoogle Scholar
  28. Thomas E, Wenzel G (1975) Z. Pflanzenzucht. 74: 77–81Google Scholar
  29. Wenzel G, Hoffmann F, Thomas E (1977) Z. Pflanzenzucht. 78: 149–155Google Scholar
  30. Yang C, Pan NS, Lin CQ (1979) Acta Bot. Sinica. 21: 378–379Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Leelavathi
    • 1
  • V. S. Reddy
    • 1
  • S. K. Sen
    • 1
  1. 1.Programme in Genetical Research, Department of BotanyBose InstituteCalcuttaIndia

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