Plant Cell Reports

, Volume 3, Issue 3, pp 102–105 | Cite as

Somatic cell genetic studies inBrassica species

I. High frequency production of haploid plants inBrassica alba (L.) H. f. & T.
  • S. Leelavathi
  • V. S. Reddy
  • S. K. Sen


In vitro culture ofBrassica alba anthers on a growth medium containing inorganics of KB5 and organics, iron, sucrose and hormones of B5 resulted in a very high response of anthers (93.75%) towards callus induction. All the calli transferred to regeneration media responded favourably even after six months of callus induction. Numerous torpedo-shaped embryoids developed in clusters at many sites from each callus mass. Secondary embryogenesis and multiple shoot formation was also observed in many cases. The number of embryoids and plantlets produced by one embryogenic anther were as high as 169.8 and 17 respectively. 87% of the regenerated plants were haploids.

Key Words

Anther culture Brassicaalba primary and secondary embryoids 



Schenk and Hildebrandt, 1972


Gamborg et al. 1968


Nitsch and Nitsch, 1969


Chu et al. 1975


Murashige and Skoog, 1962


Blaydes, 1966


Gresshoff and Doy, 1972


Keller et al. 1975



2, 4-D

2, 4-dichlorophenoxyacetic acid


chlorophenoxyacetic acid




naphthaleneacetic acid


indoleacetic acid


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Leelavathi
    • 1
  • V. S. Reddy
    • 1
  • S. K. Sen
    • 1
  1. 1.Programme in Genetical Research, Department of BotanyBose InstituteCalcuttaIndia

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