Histochemistry

, Volume 94, Issue 2, pp 113–120 | Cite as

Ultrastructural identification of human secretin cells by the immunogold technique. Their costorage of chromogranin A and serotonin

  • L. Usellini
  • G. Finzi
  • C. Riva
  • C. Capella
  • T. Mochizuki
  • C. Yanaihara
  • N. Yanaihara
  • E. Solcia
Article

Summary

We have localized secretin in a morphologically distinctive endocrine cell scattered in the epithelium covering the villi and uppermost crypts of the human duodenum and jejunum. The human secretin cell was characterized by relatively large (mean diameter 299 nm±69 SD), fairly irregular granules, the majority of which showed homogeneous distribution of secretin and chromogranin A immunolabelling in a structurally homogeneous core. Other granules had a targetoid pattern due to an inner, argyrophobe, secretin-immunoreactive body surrounded by an argyrophil, chromogranin A immunoreactive mantle. These targetoid granules represent a distinctive ultrastructural marker of the secretin cell. Secretin cell granules have been shown to react with chromogranin A antibodies and Grimelius' silver, while lacking chromogranin B immunoreactivity. About 1/3 of secretin cells also showed serotonin immunostaining.

Keywords

Secretin Endocrine Cell Motilin Gastric Inhibitory Polypeptide Gastric Inhibitory Peptide 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. Usellini
    • 1
  • G. Finzi
    • 1
  • C. Riva
    • 1
  • C. Capella
    • 1
    • 2
  • T. Mochizuki
    • 3
  • C. Yanaihara
    • 3
  • N. Yanaihara
    • 3
  • E. Solcia
    • 2
  1. 1.Histopathology Research CenterUniversity of Pavia at VareseItaly
  2. 2.Department of Human PathologyUniversity of PaviaPaviaItaly
  3. 3.Laboratory of Bioorganic ChemistryUniversity of Shizuoka School of PharmacyShizuoka-KenJapan

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